Garrine Marcelino, Mandomando Inácio, Vubil Delfino, Nhampossa Tacilta, Acacio Sozinho, Li Shan, Paulson Joseph N, Almeida Mathieu, Domman Daryl, Thomson Nicholas R, Alonso Pedro, Stine Oscar Colin
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique.
Instituto Nacional de Saúde (INS), Ministério da Saúde, Maputo, Mozambique.
PLoS Negl Trop Dis. 2017 Jun 16;11(6):e0005671. doi: 10.1371/journal.pntd.0005671. eCollection 2017 Jun.
Although cholera is a major public health concern in Mozambique, its transmission patterns remain unknown. We surveyed the genetic relatedness of 75 Vibrio cholerae isolates from patients at Manhiça District Hospital between 2002-2012 and 3 isolates from river using multilocus variable-number tandem-repeat analysis (MLVA) and whole genome sequencing (WGS). MLVA revealed 22 genotypes in two clonal complexes and four unrelated genotypes. WGS revealed i) the presence of recombination, ii) 67 isolates descended monophyletically from a single source connected to Wave 3 of the Seventh Pandemic, and iii) four clinical isolates lacking the cholera toxin gene. This Wave 3 strain persisted for at least eight years in either an environmental reservoir or circulating within the human population. Our data raises important questions related to where these isolates persist and how identical isolates can be collected years apart despite our understanding of high change rate of MLVA loci and the V. cholerae molecular clock.
尽管霍乱是莫桑比克主要的公共卫生问题，但其传播模式仍不明确。我们利用多位点可变数目串联重复序列分析（MLVA）和全基因组测序（WGS），对2002年至2012年间从曼希卡区医院患者中分离出的75株霍乱弧菌以及从河流中分离出的3株霍乱弧菌的遗传相关性进行了调查。MLVA在两个克隆复合体中揭示了22种基因型以及四种不相关的基因型。WGS揭示了：i）重组的存在；ii）67株分离株单系起源于与第七次霍乱大流行第三波相关的单一来源；iii）四株临床分离株缺乏霍乱毒素基因。这种第三波菌株在环境储存库中持续存在了至少八年，或者在人群中传播。我们的数据提出了一些重要问题，即这些分离株在哪里持续存在，以及尽管我们了解MLVA位点和霍乱弧菌分子钟的高变化率，但为何相隔数年仍能收集到相同的分离株。
