从中国本地鸡群中分离出的一种与淋巴细胞瘤相关的新型禽逆转录病毒，可显著降低SPF鸡的生长速度并导致免疫抑制。

A novel avian retrovirus associated with lymphocytoma isolated from a local Chinese flock induced significantly reduced growth and immune suppression in SPF chickens.

作者信息

Wu Xiaoping, Zhao Jinrong, Zeng Yukun, Wu Yijian, Wang Quanxi, Wu Baocheng, Huang Yifan

机构信息

Fujian Key Lab of Traditional Chinese Veterinary Medicine and Animal Health, the College of Animal Science, Fujian Agricultural and Forestry University, Fuzhou 350002, China.

出版信息

Vet Microbiol. 2017 Jun;205:34-38. doi: 10.1016/j.vetmic.2017.04.011. Epub 2017 Apr 23.

DOI:10.1016/j.vetmic.2017.04.011

PMID:28622858

Abstract

Avian Leukosis Viruses (ALVs) are associated with neoplasias, immune suppression and reduced performance in chicken flocks. In the present study, a naturally occurring recombinant strain of ALV (FJ15HT0) was isolated from an infected flock of Chinese "Hetian" chickens, and was subsequently identified as an exogenous ALV by immuno-fluorescence assay (IFA), PCR and following entire proviral DNA nucleotide sequencing. This isolate is revealed as a novel recombinant virus, lacking viral oncogenes, with the gp85 (93.4%) of subgroup B, the U3 (92.1%) and R (95.2%) region of subgroup J, the U5 (93.8%) region and 5'UTR (95.7%) of subgroup C, as well as the gp37 (90.6%) and 3' (92.2%) of ALV-E. The simulative congenital infection with this isolate in SPF chickens resulted in significant weight loss (P<0.05) and a significant reduction in the humoral immune response to the live NDV vaccine (P<0.05), but not to the inactive AIV-H vaccine (P>0.05). Foci of lymphocytomas were observed in tissues of congenitally infected chickens at 11 weeks post-hatch, demonstrating the acute oncogenicity of the isolate.

摘要

禽白血病病毒（ALVs）与鸡群中的肿瘤形成、免疫抑制及生产性能下降有关。在本研究中，从一群感染的中国“和田”鸡中分离出一株自然发生的重组ALV毒株（FJ15HT0），随后通过免疫荧光试验（IFA）、PCR及对整个前病毒DNA核苷酸测序鉴定为外源性ALV。该分离株被鉴定为一种新型重组病毒，缺乏病毒癌基因，具有B亚群的gp85（93.4%）、J亚群的U3（92.1%）和R（95.2%）区域、C亚群的U5（93.8%）区域和5'UTR（95.7%），以及E亚群的gp37（90.6%）和3'（92.2%）。用该分离株对SPF鸡进行模拟先天性感染，导致体重显著下降（P<0.05），对新城疫活疫苗的体液免疫反应显著降低（P<0.05），但对灭活禽流感H疫苗的体液免疫反应无显著影响（P>0.05）。在孵化后11周对先天性感染鸡的组织进行观察，发现有淋巴细胞瘤病灶，证明了该分离株的急性致瘤性。