Jin Rong, Liu Guorui, Zheng Minghui, Fiedler Heidelore, Jiang Xiaoxu, Yang Lili, Wu Xiaolin, Xu Yang
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
J Chromatogr A. 2017 Aug 4;1509:114-122. doi: 10.1016/j.chroma.2017.06.022. Epub 2017 Jun 10.
Isotopic dilution gas chromatography combined with high resolution mass spectrometry (GC/HRMS) has overwhelming advantages with respect to the accuracy of congener-specific ultratrace analysis of complex persistent organic pollutants (POPs) in environmental matrices. However, an isotopic dilution GC/HRMS method for analysis of chlorinated and brominated polycyclic aromatic hydrocarbons (Cl-PAHs and Br-PAHs) using C-labelled congeners as internal standards has not been established. In this study, a method for identification and quantification of 38 congeners of Cl-PAHs and Br-PAHs in atmosphere and stack gas samples from waste incinerators was developed using the isotopic dilution GC/HRMS technique. The instrumental detection limits of the GC/HRMS method ranged from 0.2pg to 1.8pg for Cl-PAH congeners, and 0.7pg to 2.7pg for Br-PAH congeners, which were about three orders of magnitude lower than those of the GC/quadrupole MS method. This new method developed was also the first to enable determination of Cl-PAH and Br-PAH homologs comprising congeners with the same molecular skeleton and chlorine or bromine substitution numbers. Among the detected congeners, seven Cl-PAH congeners and thirteen Br-PAH congeners that were abundant in the atmosphere and stack gases released from waste incinerators were firstly detected in real samples and reported using the established isotopic dilution GC/HRMS method. The developed isotopic dilution GC/HRMS is significant and needed for better studying the environmental behavior and health risk of Cl-PAHs and Br-PAHs.
同位素稀释气相色谱法结合高分辨率质谱法（GC/HRMS）在环境基质中复杂持久性有机污染物（POPs）同类物特异性超痕量分析的准确性方面具有压倒性优势。然而，尚未建立一种使用碳标记的同类物作为内标来分析氯代和溴代多环芳烃（Cl-PAHs和Br-PAHs）的同位素稀释GC/HRMS方法。在本研究中，利用同位素稀释GC/HRMS技术开发了一种用于鉴定和定量垃圾焚烧炉大气和烟道气样品中38种Cl-PAHs和Br-PAHs同类物的方法。GC/HRMS方法对Cl-PAH同类物的仪器检测限范围为0.2皮克至1.8皮克，对Br-PAH同类物的仪器检测限范围为0.7皮克至2.7皮克，比GC/四极杆质谱法的检测限低约三个数量级。所开发的这种新方法也是首次能够测定包含具有相同分子骨架和氯或溴取代数的同类物的Cl-PAH和Br-PAH同系物。在所检测到的同类物中，首次在实际样品中检测到并使用已建立的同位素稀释GC/HRMS方法报告了在垃圾焚烧炉排放的大气和烟道气中含量丰富的7种Cl-PAH同类物和13种Br-PAH同类物。所开发的同位素稀释GC/HRMS对于更好地研究Cl-PAHs和Br-PAHs的环境行为和健康风险具有重要意义且是必要的。
