Ślączka-Wilk Magdalena M, Włodarczyk Elżbieta, Kaleniecka Aleksandra, Zarzycki Paweł K
Maritime University of Szczecin, Faculty of Marine Engineering, Department of Physics and Chemistry, Waly Chrobrego 1-2, 70-500 Szczecin, Poland.
Koszalin University of Technology, Faculty of Civil Engineering, Environmental, and Geodetic Sciences, Department of Environmental Technologies and Bioanalytics, Sniadeckich 2, 75-453 Koszalin, Poland.
J AOAC Int. 2017 Jul 1;100(4):935-949. doi: 10.5740/jaoacint.17-0168. Epub 2017 Jun 16.
There is increasing interest in the development of simple analytical systems enabling the fast screening of target components in complex samples. A number of newly invented protocols are based on quasi separation techniques involving microfluidic paper-based analytical devices and/or micro total analysis systems. Under such conditions, the quantification of target components can be performed mainly due to selective detection. The main goal of this paper is to demonstrate that miniaturized planar chromatography has the capability to work as an efficient separation and quantification tool for the analysis of multiple targets within complex environmental samples isolated and concentrated using an optimized SPE method. In particular, we analyzed various samples collected from surface water ecosystems (lakes, rivers, and the Baltic Sea of Middle Pomerania in the northern part of Poland) in different seasons, as well as samples collected during key wastewater technological processes (originating from the "Jamno" wastewater treatment plant in Koszalin, Poland). We documented that the multiple detection of chromatographic spots on RP-18W microplates-under visible light, fluorescence, and fluorescence quenching conditions, and using the visualization reagent phosphomolybdic acid-enables fast and robust sample classification. The presented data reveal that the proposed micro-TLC system is useful, inexpensive, and can be considered as a complementary method for the fast control of treated sewage water discharged by a municipal wastewater treatment plant, particularly for the detection of low-molecular mass micropollutants with polarity ranging from estetrol to progesterone, as well as chlorophyll-related dyes. Due to the low consumption of mobile phases composed of water-alcohol binary mixtures (less than 1 mL/run for the simultaneous separation of up to nine samples), this method can be considered an environmentally friendly and green chemistry analytical tool. The described analytical protocol can be complementary to those involving classical column chromatography (HPLC) or various planar microfluidic devices.
人们对开发能够快速筛选复杂样品中目标成分的简单分析系统越来越感兴趣。许多新发明的方案基于准分离技术，涉及基于微流控纸的分析装置和/或微全分析系统。在这种情况下，目标成分的定量主要通过选择性检测来实现。本文的主要目的是证明小型化平面色谱有能力作为一种高效的分离和定量工具，用于分析使用优化的固相萃取方法分离和浓缩的复杂环境样品中的多种目标物。特别是，我们分析了不同季节从地表水生态系统（湖泊、河流以及波兰北部中波美拉尼亚的波罗的海）采集的各种样品，以及关键废水处理工艺过程中采集的样品（来自波兰科沙林的“亚姆诺”废水处理厂）。我们记录到，在RP - 18W微孔板上，在可见光、荧光和荧光猝灭条件下，并使用可视化试剂磷钼酸，对色谱斑点进行多重检测能够实现快速且可靠的样品分类。所呈现的数据表明，所提出的微型薄层色谱系统实用、廉价，可被视为一种用于快速控制城市污水处理厂排放的处理后污水的补充方法，尤其适用于检测极性范围从雌三醇到孕酮以及叶绿素相关染料的低分子量微污染物。由于由水 - 醇二元混合物组成的流动相消耗低（同时分离多达九个样品时每次运行小于1 mL），该方法可被视为一种环境友好的绿色化学分析工具。所描述的分析方案可以补充那些涉及经典柱色谱（HPLC）或各种平面微流控装置的方案。
