Torres-Duarte Cristina, Ramos-Torres Karla M, Rahimoff René, Cherr Gary N
Bodega Marine Laboratory, University of California Davis, Bodega Bay, CA, USA.
Department of Chemistry, University of California Berkeley, Berkeley, CA, USA.
Aquat Toxicol. 2017 Aug;189:134-141. doi: 10.1016/j.aquatox.2017.05.008. Epub 2017 Jun 1.
The effects of exposure to either soluble copper (copper sulfate) or copper oxide nanoparticles (nano-CuO) during specific early developmental stages of sea urchin embryos were analyzed. Soluble copper caused significant malformations in embryos (skeletal malformations, delayed development or gut malformations) when present at any given stage, while cleavage stage was the most sensitive to nano-CuO exposure causing skeletal malformations and decreased total antioxidant capacity. The stage specificity was linked to higher endocytic activity during the first hours of development that leads to higher accumulation of copper in specific cells critical for development. Results indicate that nano-CuO results in higher accumulation of copper inside of embryos and this intracellular copper is more persistent as compared to soluble copper. The possible implications later in development are discussed.
分析了在海胆胚胎特定早期发育阶段暴露于可溶性铜（硫酸铜）或氧化铜纳米颗粒（纳米CuO）的影响。当在任何给定阶段存在时，可溶性铜会导致胚胎出现明显的畸形（骨骼畸形、发育延迟或肠道畸形），而卵裂期对纳米CuO暴露最为敏感，会导致骨骼畸形和总抗氧化能力下降。阶段特异性与发育最初几小时内较高的内吞活性有关，这导致铜在对发育至关重要的特定细胞中积累更多。结果表明，纳米CuO导致胚胎内铜的积累更多，并且与可溶性铜相比，这种细胞内铜更持久。讨论了其在后期发育中可能产生的影响。
