Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, School of Environment, Jinan University, Guangzhou 510632, China.
Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, School of Environment, Jinan University, Guangzhou 510632, China.
Sci Total Environ. 2017 Dec 15;603-604:66-76. doi: 10.1016/j.scitotenv.2017.06.040. Epub 2017 Jun 23.
Ciprofloxacin (CIP) is one of most used quinolone antibiotics detected frequently in agricultural soils and vegetables. In the present study, variations in microbial community and CIP removal in rhizospheric soils between two cultivars of Brassica parachinensis L. that accumulate higher and lower CIP (Sijiu and Cutai, respectively) were investigated under CIP stress (0mg/kg in CK, 2.94mg/kg in T1, and 67.11mg/kg in T2). The removal rates of CIP in rhizospheric soils of cultivar Sijiu were higher than those of cultivar Cutai, with a significant difference in T2 (48.7%>39.4%, P<0.05). The pyrosequencing of 16S rRNA and ITS gene indicated that the microbial diversity and community structure in rhizospheric soils of the two cultivars varied significantly. Spirochaeta and Trichosporon might be associated with CIP degradation, and higher relative abundances of Trichosporon in rhizoshperic soils of cultivar Sijiu might be responsible for higher CIP removal. Fourteen bacterial genera and ten fungal genera were screened as potential biomarkers for CIP removal process. The community level physiological profiling in rhizospheric soils of the two cultivars under CIP stress differed significantly, and more C substrates that favored CIP removal were observed in rhizoshperic soils of cultivar Sijiu. Our results demonstrate that variations in microbial community and the utilization of C substrates played important roles in differring the CIP removal in rhizospheric soils between the two cultivars.
环丙沙星（CIP）是农业土壤和蔬菜中经常检测到的最常用的喹诺酮类抗生素之一。本研究在 CIP 胁迫下（CK 中为 0mg/kg，T1 中为 2.94mg/kg，T2 中为 67.11mg/kg），研究了两种芥蓝（Sijiu 和 Cutai）根际土壤中微生物群落和 CIP 去除率的变化，这两种芥蓝分别积累了较高和较低的 CIP。Sijiu 品种根际土壤中 CIP 的去除率高于 Cutai 品种，T2 时差异显著（48.7%>39.4%，P<0.05）。16S rRNA 和 ITS 基因的焦磷酸测序表明，两种品种根际土壤的微生物多样性和群落结构差异显著。螺旋体和 Trichosporon 可能与 CIP 降解有关，Sijiu 品种根际土壤中 Trichosporon 的相对丰度较高，可能是 CIP 去除率较高的原因。筛选出 14 个细菌属和 10 个真菌属作为 CIP 去除过程的潜在生物标志物。两种品种根际土壤在 CIP 胁迫下的群落水平生理谱差异显著，Sijiu 品种根际土壤中观察到更多有利于 CIP 去除的 C 底物。研究结果表明，微生物群落的变化和 C 底物的利用在两种品种根际土壤中 CIP 去除的差异中起重要作用。
