Schlemper Thiago R, Leite Márcio F A, Lucheta Adriano R, Shimels Mahdere, Bouwmeester Harro J, van Veen Johannes A, Kuramae Eiko E
Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, 6708 PB Wageningen, Netherlands.
Department of Biology, Leiden University, 2333 BE Leiden, The Netherlands.
FEMS Microbiol Ecol. 2017 Aug 1;93(8). doi: 10.1093/femsec/fix096.
Plant genotype selects the rhizosphere microbiome. The success of plant-microbe interactions is dependent on factors that directly or indirectly influence the plant rhizosphere microbial composition. We investigated the rhizosphere bacterial community composition of seven different sorghum cultivars in two different soil types (abandoned (CF) and agricultural (VD)). The rhizosphere bacterial community was evaluated at four different plant growth stages: emergence of the second (day 10) and third leaves (day 20), the transition between the vegetative and reproductive stages (day 35), and the emergence of the last visible leaf (day 50). At early stages (days 10 and 20), the sorghum rhizosphere bacterial community composition was mainly driven by soil type, whereas at late stages (days 35 and 50), the bacterial community composition was also affected by the sorghum genotype. Although this effect of sorghum genotype was small, different sorghum cultivars assembled significantly different bacterial community compositions. In CF soil, the striga-resistant cultivar had significantly higher relative abundances of Acidobacteria GP1, Burkholderia, Cupriavidus (Burkholderiaceae), Acidovorax and Albidiferax (Comamonadaceae) than the other six cultivars. This study is the first to simultaneously investigate the contributions of plant genotype, plant growth stage and soil type in shaping sorghum rhizosphere bacterial community composition.
植物基因型选择根际微生物群落。植物与微生物相互作用的成功取决于直接或间接影响植物根际微生物组成的因素。我们研究了两种不同土壤类型（弃耕地（CF）和农田（VD））中七个不同高粱品种的根际细菌群落组成。在四个不同的植物生长阶段评估根际细菌群落：第二片叶（第10天）和第三片叶出现（第20天）、营养生长和生殖生长阶段之间的过渡（第35天）以及最后一片可见叶出现（第50天）。在早期阶段（第10天和第20天），高粱根际细菌群落组成主要受土壤类型驱动，而在后期阶段（第35天和第50天），细菌群落组成也受高粱基因型影响。尽管高粱基因型的这种影响较小，但不同高粱品种组装出的细菌群落组成明显不同。在CF土壤中，抗列当品种的嗜酸菌纲GP1、伯克霍尔德菌属、贪铜菌属（伯克霍尔德菌科）、嗜酸菌属和白杆菌属（丛毛单胞菌科）的相对丰度显著高于其他六个品种。本研究首次同时调查了植物基因型、植物生长阶段和土壤类型对塑造高粱根际细菌群落组成的作用。
