Castells Matías, Victoria Matías, Colina Rodney, Musto Héctor, Cristina Juan
Laboratorio de Virología Molecular, Sede Salto, Centro Universitario Regional Litoral Norte, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay.
Laboratorio de Organización y Evolución del Genoma, Unidad de Genómica Evolutiva, Instituto de Biología, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay.
Virol J. 2017 Jun 17;14(1):115. doi: 10.1186/s12985-017-0780-y.
Bovine coronavirus (BCoV) belong to the genus Betacoronavirus of the family Coronaviridae. BCoV are widespread around the world and cause enteric or respiratory infections among cattle, leading to important economic losses to the beef and dairy industry worldwide. To study the relation of codon usage among viruses and their hosts is essential to understand host-pathogen interaction, evasion from host's immune system and evolution.
We performed a comprehensive analysis of codon usage and composition of BCoV.
The global codon usage among BCoV strains is similar. Significant differences of codon preferences in BCoV genes in relation to codon usage of Bos taurus host genes were found. Most of the highly frequent codons are U-ending. G + C compositional constraint and dinucleotide composition also plays a role in the overall pattern of BCoV codon usage.
The results of these studies revealed that mutational bias is a leading force shaping codon usage in this virus. Additionally, relative dinucleotide frequencies, geographical distribution, and evolutionary processes also influenced the codon usage pattern.
牛冠状病毒（BCoV）属于冠状病毒科β冠状病毒属。BCoV在全球广泛传播，可引起牛的肠道或呼吸道感染，给全球牛肉和乳制品行业造成重大经济损失。研究病毒与其宿主之间的密码子使用关系对于理解宿主-病原体相互作用、逃避宿主免疫系统以及进化至关重要。
我们对BCoV的密码子使用和组成进行了全面分析。
BCoV毒株之间的全球密码子使用情况相似。发现BCoV基因中的密码子偏好与牛宿主基因的密码子使用存在显著差异。大多数高频密码子以U结尾。G+C组成限制和二核苷酸组成也在BCoV密码子使用的总体模式中起作用。
这些研究结果表明，突变偏向是塑造该病毒密码子使用的主要力量。此外，相对二核苷酸频率、地理分布和进化过程也影响了密码子使用模式。
