Cristina Juan, Moreno Pilar, Moratorio Gonzalo, Musto Héctor
Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay.
Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay.
Virus Res. 2015 Jan 22;196:87-93. doi: 10.1016/j.virusres.2014.11.005. Epub 2014 Nov 14.
Ebola virus (EBOV) is a member of the family Filoviridae and its genome consists of a 19-kb, single-stranded, negative sense RNA. EBOV is subdivided into five distinct species with different pathogenicities, being Zaire ebolavirus (ZEBOV) the most lethal species. The interplay of codon usage among viruses and their hosts is expected to affect overall viral survival, fitness, evasion from host's immune system and evolution. In the present study, we performed comprehensive analyses of codon usage and composition of ZEBOV. Effective number of codons (ENC) indicates that the overall codon usage among ZEBOV strains is slightly biased. Different codon preferences in ZEBOV genes in relation to codon usage of human genes were found. Highly preferred codons are all A-ending triplets, which strongly suggests that mutational bias is a main force shaping codon usage in ZEBOV. Dinucleotide composition also plays a role in the overall pattern of ZEBOV codon usage. ZEBOV does not seem to use the most abundant tRNAs present in the human cells for most of their preferred codons.
埃博拉病毒（EBOV）是丝状病毒科的成员，其基因组由一条19kb的单链负义RNA组成。EBOV可细分为五个具有不同致病性的不同物种，其中扎伊尔埃博拉病毒（ZEBOV）是最致命的物种。病毒与其宿主之间密码子使用的相互作用预计会影响病毒的整体生存、适应性、逃避宿主免疫系统的能力以及进化。在本研究中，我们对ZEBOV的密码子使用和组成进行了全面分析。有效密码子数（ENC）表明ZEBOV毒株之间的整体密码子使用略有偏向。发现ZEBOV基因中与人类基因密码子使用相关的不同密码子偏好。高度偏好的密码子都是以A结尾的三联体，这强烈表明突变偏向是塑造ZEBOV密码子使用的主要力量。二核苷酸组成也在ZEBOV密码子使用的整体模式中起作用。ZEBOV似乎没有将人类细胞中最丰富的tRNA用于其大多数偏好的密码子。
