School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
Bioresour Technol. 2017 Dec;245(Pt B):1603-1609. doi: 10.1016/j.biortech.2017.06.008. Epub 2017 Jun 16.
In this study, the optimum induction and reaction conditions, and fermentation process of producing Ala-Gln by E. coli Origami 2 overexpressing α-amino acid ester acyltransferase (OPA) were investigated. Besides, the Ala-Gln synthesis by OPA achieved the maximum molar yield of 94.7% and productivity of 1.89g/L/min due to the extremely high enzyme activity. On this basis, repeated-cycle batch fermentation to produce Ala-Gln indicated that OPA could maintain high Ala-Gln yields and enzyme stabilities after several cell recycling. Consequently, the cost-efficient and environmentally friendly approach for Ala-Gln production by recycling OPA makes a great contribution to further industrial-scale applications.
在这项研究中，我们研究了通过过表达α-氨基酸酯酰基转移酶（OPA）的大肠杆菌 Origami 2 生产 Ala-Gln 的最佳诱导和反应条件以及发酵工艺。此外，由于酶活极高，OPA 合成 Ala-Gln 的最大摩尔产率达到 94.7%，生产强度达到 1.89g/L/min。在此基础上，通过重复循环分批发酵生产 Ala-Gln，表明 OPA 在多次细胞回收后仍能保持高产 Ala-Gln 和高酶稳定性。因此，OPA 回收生产 Ala-Gln 的这种经济高效且环保的方法为进一步的工业化应用做出了巨大贡献。
