基于神经氨酸酶的重组病毒样颗粒可保护雪貂免受致死性甲型禽流感病毒（H5N1）感染。
Neuraminidase-based recombinant virus-like particles protect against lethal avian influenza A(H5N1) virus infection in ferrets.
作者信息
Smith Gale E, Sun Xiangjie, Bai Yaohui, Liu Ye V, Massare Michael J, Pearce Melissa B, Belser Jessica A, Maines Taronna R, Creager Hannah M, Glenn Gregory M, Flyer David, Pushko Peter, Levine Min Z, Tumpey Terrence M
机构信息
Novavax, Inc., 20 Firstfield, Gaithersburg, MD 20878, USA.
Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
出版信息
Virology. 2017 Sep;509:90-97. doi: 10.1016/j.virol.2017.06.006. Epub 2017 Jun 16.
Abstract
Avian influenza A (H5N1) viruses represent a growing threat for an influenza pandemic. The presence of widespread avian influenza virus infections further emphasizes the need for vaccine strategies for control of pre-pandemic H5N1 and other avian influenza subtypes. Influenza neuraminidase (NA) vaccines represent a potential strategy for improving vaccines against avian influenza H5N1 viruses. To evaluate a strategy for NA vaccination, we generated a recombinant influenza virus-like particle (VLP) vaccine comprised of the NA protein of A/Indonesia/05/2005 (H5N1) virus. Ferrets vaccinated with influenza N1 NA VLPs elicited high-titer serum NA-inhibition (NI) antibody titers and were protected from lethal challenge with A/Indonesia/05/2005 virus. Moreover, N1-immune ferrets shed less infectious virus than similarly challenged control animals. In contrast, ferrets administered control N2 NA VLPs were not protected against H5N1 virus challenge. These results provide support for continued development of NA-based vaccines against influenza H5N1 viruses.
摘要
甲型禽流感（H5N1）病毒对流感大流行构成日益严重的威胁。广泛存在的禽流感病毒感染进一步凸显了制定疫苗策略以控制大流行前H5N1及其他禽流感亚型的必要性。流感神经氨酸酶（NA）疫苗是改进针对H5N1禽流感病毒疫苗的一种潜在策略。为评估NA疫苗接种策略，我们制备了一种重组流感病毒样颗粒（VLP）疫苗，其由A/印度尼西亚/05/2005（H5N1）病毒的NA蛋白组成。用流感N1 NA VLP疫苗接种的雪貂产生了高滴度的血清NA抑制（NI）抗体滴度，并对A/印度尼西亚/05/2005病毒的致死性攻击具有抵抗力。此外，N1免疫的雪貂排出的感染性病毒比同样受到攻击的对照动物少。相比之下，接种对照N2 NA VLP的雪貂对H5N1病毒攻击没有抵抗力。这些结果为继续研发针对H5N1禽流感病毒的基于NA的疫苗提供了支持。
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