吖啶橙染色在检测阳性血培养物中细菌的应用。
Utility of Acridine Orange staining for detection of bacteria from positive blood cultures.
作者信息
Neeraja M, Lakshmi V, Padmasri C, Padmaja K
机构信息
Dept. of Microbiology, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
Dept. of Microbiology, Nizam's Institute of Medical Sciences, Hyderabad, Telangana, India.
出版信息
J Microbiol Methods. 2017 Aug;139:215-217. doi: 10.1016/j.mimet.2017.06.014. Epub 2017 Jun 15.
The diagnostic performance of AO stain was evaluated for the detection of bacteria and or fungi from positive blood cultures. The sensitivity of Gram stain (GS) was 98.26% while Acridine Orange (AO) stain proved to be more sensitive (100%) with a Positive and Negative Predictive Value of 100% each. The specificity of both the stains was 100%. Overall agreement between the two stains was 98.23% (688/700). The organisms that were missed by GS and positive by AO were Candida species (Sutton, 2006) and Gram negative bacilli (GNB) (Sutton, 2006). Sensitivity of GS was 82.35% and AO was 100% among mixed cultures. Immediate reporting of the results of AO stain would have a significant impact on clinical management of patients with serious blood stream infections.
评估吖啶橙（AO）染色法在从阳性血培养物中检测细菌和/或真菌方面的诊断性能。革兰氏染色（GS）的敏感性为98.26%，而吖啶橙（AO）染色法被证明更敏感（100%），其阳性预测值和阴性预测值均为100%。两种染色法的特异性均为100%。两种染色法的总体一致性为98.23%（688/700）。革兰氏染色法漏检而吖啶橙染色法呈阳性的微生物为念珠菌属（萨顿，2006年）和革兰氏阴性杆菌（GNB）（萨顿，2006年）。在混合培养物中，革兰氏染色法的敏感性为82.35%，吖啶橙染色法的敏感性为100%。立即报告吖啶橙染色的结果将对严重血流感染患者的临床管理产生重大影响。
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