Pal Siddhartha, Kundu Anirban, Banerjee Tirtha Das, Mohapatra Balaram, Roy Ajoy, Manna Riddha, Sar Pinaki, Kazy Sufia K
Department of Biotechnology, National Institute of Technology Durgapur, 713209, India.
Department of Biotechnology, Indian Institute of Technology Kharagpur, 721302, India.
Genomics. 2017 Oct;109(5-6):374-382. doi: 10.1016/j.ygeno.2017.06.002. Epub 2017 Jun 15.
Franconibacter pulveris strain DJ34, isolated from Duliajan oil fields, Assam, was characterized in terms of its taxonomic, metabolic and genomic properties. The bacterium showed utilization of diverse petroleum hydrocarbons and electron acceptors, metal resistance, and biosurfactant production. The genome (4,856,096bp) of this strain contained different genes related to the degradation of various petroleum hydrocarbons, metal transport and resistance, dissimilatory nitrate, nitrite and sulfite reduction, chemotaxy, biosurfactant synthesis, etc. Genomic comparison with other Franconibacter spp. revealed higher abundance of genes for cell motility, lipid transport and metabolism, transcription and translation in DJ34 genome. Detailed COG analysis provides deeper insights into the genomic potential of this organism for degradation and survival in oil-contaminated complex habitat. This is the first report on ecophysiology and genomic inventory of Franconibacter sp. inhabiting crude oil rich environment, which might be useful for designing the strategy for bioremediation of oil contaminated environment.
从阿萨姆邦杜利亚詹油田分离出的粉状弗兰科尼氏菌菌株DJ34，对其分类学、代谢和基因组特性进行了表征。该细菌显示出对多种石油烃和电子受体的利用、金属抗性以及生物表面活性剂的产生。该菌株的基因组（4,856,096bp）包含与各种石油烃降解、金属转运和抗性、异化硝酸盐、亚硝酸盐和亚硫酸盐还原、趋化性、生物表面活性剂合成等相关的不同基因。与其他弗兰科尼氏菌属的基因组比较显示，DJ34基因组中细胞运动性、脂质转运和代谢、转录和翻译相关基因的丰度更高。详细的COG分析为该生物体在受油污染的复杂生境中进行降解和生存的基因组潜力提供了更深入的见解。这是关于栖息在富含原油环境中的弗兰科尼氏菌生态生理学和基因组清单的首次报告，这可能有助于设计石油污染环境生物修复策略。
