Kakeshpour Tayebeh, Monfared Sajad Rashidi, Ebrahimi Amin, Beyraghdar Kashkooli Arman, Ebrahimie Esmaeil
Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, 14977-13111, Iran.
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Shahrood, 3619995161, Iran.
Gene Expr Patterns. 2017 Nov;25-26:76-84. doi: 10.1016/j.gep.2017.06.005. Epub 2017 Jun 15.
Salinity is among the most important abiotic stresses affecting crop production throughout the earth. Halophyte plants can sustain high salinity levels, therefore elucidating molecular mechanisms underlying their salinity resistance is beneficial for crop improvement. Aeluropus littoralis, a halophyte weed, is a great genetic resource for this purpose. Isolated expressed sequence taq (EST) sequences from A. littoralis under salinity stress, have given us the chance to find and analyze transcripts of genes involved in response to salinity. Transcriptome analyses indicated the expression levels of mRNAs corresponding to 10 of sequences were increased under treatments. All mRNAs were significantly induced under salt treatment with the highest peaks observed at different hours of treatments. Moreover, the full-length cDNA of vacuolar H-pyrophosphatase (VP) was isolated utilizing 3' and 5' rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR) and characterized (GenBank accession number of KT253223.1). The extracted full-length of VP was 2732 bp, which contained ORF of 2292 bp encoding 763 amino acids.
盐度是影响全球作物产量的最重要非生物胁迫之一。盐生植物能够耐受高盐度水平，因此阐明其耐盐性的分子机制有助于作物改良。滨海刺碱蓬作为一种盐生杂草，是实现这一目标的优质遗传资源。从盐胁迫下的滨海刺碱蓬中分离出表达序列标签（EST）序列，这使我们有机会找到并分析参与盐胁迫响应的基因转录本。转录组分析表明，在处理条件下，与10个序列相对应的mRNA表达水平有所增加。在盐处理下，所有mRNA均被显著诱导，在不同处理时间观察到最高峰。此外，利用3'和5' cDNA末端快速扩增聚合酶链反应（RACE-PCR）分离并鉴定了液泡H + -焦磷酸酶（VP）的全长cDNA（GenBank登录号为KT253223.1）。提取的VP全长为2732 bp，其中包含2292 bp的开放阅读框，编码763个氨基酸。
