Kong Fan-Fei, Li Da, Yang Hui, Ma Jian, Pan Xin, Liu Hong-Xiang, Huo Jia-Ning, Ma Xiao-Xin
Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
Biochem Biophys Res Commun. 2017 Aug 19;490(2):506-513. doi: 10.1016/j.bbrc.2017.06.070. Epub 2017 Jun 15.
Stem cells play a critical role in endometrial cancer progression. However, the current methodologies used to isolate endometrial cancer stem cells (ECSCs) remain unsatisfactory. The ECSCs were isolated by serumfree suspension cultivation. The stem cells-related genes CD44, CD133, Oct4, Sox2 and Nanog were analyzed, and the biological behaviour of ECSCs was evaluated in vitro and vivo. The results suggest that (i) serumfree suspension cultivation is non-toxic and a convenient way for isolating the ECSCs, and is not limited to specific surface markers; (ii) Ishikawa cells can be used as an effective source of ECSCs, and the obtained ECSCs expressing the pluripotent stem cells markers CD44, CD133, Oct4, Sox2, and Nanog; (iii) ECSCs originated from Ishikawa cells showed an increased ability to invasion and metastasis in vitro, and exhibited a high proliferative capacity and pluripotency in vivo and vitro. These findings indicate that serumfree suspension cultivation is an effective method for isolating ECSCs from Ishikawa cells, and the obtained ECSCs are tumorigenic and display stem cell-like properties.
干细胞在子宫内膜癌进展中发挥着关键作用。然而，目前用于分离子宫内膜癌干细胞（ECSCs）的方法仍不尽人意。通过无血清悬浮培养分离出ECSCs。分析了干细胞相关基因CD44、CD133、Oct4、Sox2和Nanog，并在体外和体内评估了ECSCs的生物学行为。结果表明：（i）无血清悬浮培养无毒，是分离ECSCs的便捷方法，且不受特定表面标志物的限制；（ii）Ishikawa细胞可作为ECSCs的有效来源，所获得的ECSCs表达多能干细胞标志物CD44、CD133、Oct4、Sox2和Nanog；（iii）源自Ishikawa细胞的ECSCs在体外表现出增强的侵袭和转移能力，在体内和体外均表现出高增殖能力和多能性。这些发现表明，无血清悬浮培养是从Ishikawa细胞中分离ECSCs的有效方法，所获得的ECSCs具有致瘤性并表现出干细胞样特性。
