Anzola-Rojas Mélida Del Pilar, Gonçalves da Fonseca Samantha, Canedo da Silva Cynthia, Maia de Oliveira Valeria, Zaiat Marcelo
Laboratory of Biological Processes, Center for Research, Development and Innovation in Environmental Engineering, São Carlos School of Engineering, University of São Paulo 1100, João Dagnone Ave., Santa Angelina, 13563-120 São Carlos, São Paulo, Brazil.
Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University, CP 6171, CEP 13081-970 Campinas, São Paulo, Brazil.
Biotechnol Rep (Amst). 2014 Nov 4;5:46-54. doi: 10.1016/j.btre.2014.10.010. eCollection 2015 Mar.
This study assessed the effect of the carbon/nitrogen (C/N) ratio on the hydrogen production from sucrose-based synthetic wastewater in upflow fixed-bed anaerobic reactors. C/N ratios of 40, 90, 140, and 190 (g C/g N) were studied using sucrose and urea as the carbon and nitrogen sources, respectively. An optimum hydrogen yield of 3.5 mol H mol sucrose was obtained for a C/N ratio of 137 by means of mathematical adjustment. For all C/N ratios, the sucrose removal efficiency reached values greater than 80% and was stable after the transient stage. However, biogas production was not stable at all C/N ratios as a consequence of the continuous decreasing of the specific organic loading rate (SOLR) when the biomass accumulated in the fixed-bed, causing the proliferation of H-consuming microorganisms. It was found that the application of a constant SOLR of 6.0 g sucrose g VSS d stabilized the system.
本研究评估了碳氮（C/N）比对上流式固定床厌氧反应器中基于蔗糖的合成废水产氢的影响。分别使用蔗糖和尿素作为碳源和氮源，研究了40、90、140和190（g C/g N）的C/N比。通过数学调整，C/N比为137时获得了3.5 mol H/mol蔗糖的最佳产氢量。对于所有C/N比，蔗糖去除效率均达到80%以上，且在过渡阶段后保持稳定。然而，由于固定床中生物质积累时比有机负荷率（SOLR）持续下降，导致消耗氢气的微生物增殖，所有C/N比下的沼气产量都不稳定。研究发现，应用6.0 g蔗糖/g VSS/d的恒定SOLR可使系统稳定。
