Lloyd C Chad, Boyce Matthew W, Lockett Matthew R
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
Curr Protoc Chem Biol. 2017 Jun 19;9(2):75-95. doi: 10.1002/cpch.22.
To elucidate the chemical and environmental conditions that promote invasion of cancer cells, an assay is needed in which the chemical landscape of a tumor-like environment can be experimentally manipulated and probed. The three-dimensional paper-based invasion assays described here simulate poorly vascularized tissue and allow the invasion of cancerous cells to be visualized and quantified. These cultures are easy to assemble and allow multiple invasion assays to be performed in parallel. By using different materials to control gradients formed across the culture, the chemotactic potential of small molecules can be evaluated in a more representative tissue microenvironment. © 2017 by John Wiley & Sons, Inc.
为了阐明促进癌细胞侵袭的化学和环境条件，需要一种能够对肿瘤样环境的化学环境进行实验性操作和探测的分析方法。本文所述的基于纸张的三维侵袭分析方法模拟了血管化不良的组织，并能对癌细胞的侵袭进行可视化和定量分析。这些培养物易于组装，可同时进行多个侵袭分析。通过使用不同材料来控制培养物中形成的梯度，可以在更具代表性的组织微环境中评估小分子的趋化潜力。© 2017约翰威立国际出版公司。
