Wang Lin-Xiang, Zhou Ying, Fu Jing-Jing, Lu Zhisong, Yu Ling
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China.
Guangan Changming Research Institute for Advanced Industrial Technology, Guangan 638500, China.
Micromachines (Basel). 2018 Dec 14;9(12):660. doi: 10.3390/mi9120660.
Cancer cell metastasis has been recognized as one hallmark of malignant tumor progression; thus, measuring the motility of cells, especially tumor cell migration, is important for evaluating the therapeutic effects of anti-tumor drugs. Here, we used a paper-based cell migration platform to separate and isolate cells according to their distinct motility. A multi-layer cells-in-gels-in-paper (CiGiP) stack was assembled. Only a small portion of DU 145 prostate cancer cells seeded in the middle layer could successfully migrate into the top and bottom layers of the stack, showing heterogeneous motility. The cells with distinct migration were isolated for further analysis. Quantitative PCR assay results demonstrated that cells with higher migration potential had increased expression of the ALDH1A1, SRY (sex-determining region Y)-box 2, NANOG, and octamer-binding transcription 4. Increased doxorubicin tolerance was also observed in cells that migrated through the CiGiP layers. In summary, the separation and characterization of prostate cancer cell subtype can be achieved by using the multi-layer CiGiP cell migration platform.
癌细胞转移已被公认为恶性肿瘤进展的一个标志；因此，测量细胞的运动性，尤其是肿瘤细胞的迁移，对于评估抗肿瘤药物的治疗效果很重要。在此，我们使用了一种基于纸的细胞迁移平台，根据细胞不同的运动性对细胞进行分离和隔离。组装了一个多层的凝胶包埋细胞-纸（CiGiP）堆栈。接种在中间层的一小部分DU 145前列腺癌细胞能够成功迁移到堆栈的顶层和底层，显示出异质性运动性。分离出具有不同迁移能力的细胞进行进一步分析。定量PCR检测结果表明，具有较高迁移潜能的细胞中醛脱氢酶1A1、性别决定区Y框2、NANOG和八聚体结合转录因子4的表达增加。在通过CiGiP层迁移的细胞中还观察到阿霉素耐受性增加。总之，使用多层CiGiP细胞迁移平台可以实现前列腺癌细胞亚型的分离和表征。
