Saguie Bianca O, Romana-Souza Bruna, Martins Rayssa L, Monte-Alto-Costa Andréa
Department of Histology and Embryology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Wound Repair Regen. 2017 Aug;25(4):641-651. doi: 10.1111/wrr.12556. Epub 2017 Jun 30.
Stress-induced prolonged inflammation impairs cutaneous wound healing. Exercise may inhibit this effect via an anti-inflammatory mechanism. Our aim was to investigate the effect of moderate exercise on skin wound healing in chronically stressed mice. Mice were trained five times per week on a treadmill or received no training. Mice underwent daily rotational stress from the 6th week until euthanasia. During the 8th week, two wounds were created in the dorsum and collected 10 days later. A control group only received wounds. Exercise was performed prior to and simultaneous with stress for 2 weeks or only prior to stress. Stress increased normetanephrine levels 10 days after wounding, resulting in an increased amount of inflammatory cells and reduced expression of inflammatory cytokines as well as angiogenesis, myofibroblast differentiation and matrix deposition. Concomitant exercise and stress potentiated these effects, intensifying the delayed wound contraction. When exercise was performed only prior to stress, however, the mice showed reduced inflammatory cells in granulation tissue 10 days after wounding and improved wound healing compared with animals with exercise and concomitant stress. Moderate exercise in association with stress potentiates the stress effect; however, when exercise was performed prior to stress, wound healing was improved.
应激诱导的长期炎症会损害皮肤伤口愈合。运动可能通过抗炎机制抑制这种效应。我们的目的是研究适度运动对慢性应激小鼠皮肤伤口愈合的影响。小鼠每周在跑步机上训练五次或不接受训练。从第6周直到安乐死，小鼠每天接受旋转应激。在第8周时，在小鼠背部制造两个伤口，并在10天后收集样本。对照组仅接受伤口处理。运动在应激之前进行2周或在应激同时进行2周，或者仅在应激之前进行。伤口损伤10天后，应激使去甲变肾上腺素水平升高，导致炎症细胞数量增加，炎症细胞因子表达减少，同时血管生成、肌成纤维细胞分化和基质沉积也减少。运动与应激同时存在会增强这些效应，加剧伤口收缩延迟。然而，当仅在应激之前进行运动时，与运动和应激同时存在的动物相比，受伤10天后小鼠肉芽组织中的炎症细胞减少，伤口愈合得到改善。适度运动与应激同时存在会增强应激效应；然而，当在应激之前进行运动时，伤口愈合得到改善。
