Laboratório de Reparo Tecidual Cutâneo, Centro Biomédico, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
Braz J Med Biol Res. 2011 Oct;44(10):1025-35. doi: 10.1590/s0100-879x2011007500115. Epub 2011 Sep 2.
Physical training influences the cells and mediators involved in skin wound healing. The objective of this study was to determine the changes induced by different intensities of physical training in mouse skin wound healing. Ninety male C57BL6 mice (8 weeks old, 20-25 g) were randomized into three physical training groups: moderate (70% VO2max), high (80% VO2max), and strenuous intensity (90% VO2max). Animals trained on a motorized treadmill for 8 weeks (E lesion: physical training until the day of excisional lesion, N = 10) or 10 weeks (E euthan: physical training for 2 additional weeks after excisional lesion until euthanasia, N = 10), five times/week, for 45 min. Control groups (CG) trained on the treadmill three times/week only for 5 min (N = 10). In the 8th week, mice were anesthetized, submitted to a dorsal full-thickness excisional wound of 1 cm², and sacrificed 14 days after wounding. Wound areas were measured 4, 7, and 14 days after wounding to evaluate contraction (d4, d7 and d14) and re-epithelialization (d14). Fragments of lesion and adjacent skin were processed and submitted to routine histological staining. Immunohistochemistry against alpha-smooth muscle actin (α-SMA) was performed. Moderate-intensity training (M) until lesion (M/E lesion) led to better wound closure 7 days after wounding compared to controls and M/E euthan (P < 0.05), and both moderate-intensity groups showed better re-epithelialization rates than controls (M/E lesion = 85.9%, M/E euthan = 96.4% and M/CG = 79.9%; P < 0.05). Sections of M/E lesion and M/E euthan groups stained with hematoxylin-eosin, Picrosirius red and α-SMA showed the most mature granulation tissues among all trained groups and controls. Thus, moderate-intensity physical training improves skin wound healing.
身体训练会影响参与皮肤伤口愈合的细胞和介质。本研究的目的是确定不同强度的身体训练对小鼠皮肤伤口愈合的诱导变化。将 90 只雄性 C57BL6 小鼠（8 周龄，20-25g）随机分为三组身体训练组：中等强度（70%VO2max）、高强度（80%VO2max）和剧烈强度（90%VO2max）。动物在电动跑步机上训练 8 周（E 损伤：直到切除损伤的那天进行身体训练，N=10）或 10 周（E 安乐死：切除损伤后再进行 2 周的身体训练，直到安乐死，N=10），每周 5 次，每次 45 分钟。对照组（CG）仅在跑步机上每周训练 3 次，每次 5 分钟（N=10）。在第 8 周，麻醉小鼠，进行背部全层切除伤口 1cm²，并在受伤后 14 天处死。在受伤后 4、7 和 14 天测量伤口面积，以评估收缩（d4、d7 和 d14）和再上皮化（d14）。对损伤和相邻皮肤的片段进行处理并进行常规组织学染色。进行针对α-平滑肌肌动蛋白（α-SMA）的免疫组织化学染色。与对照组和 M/E 安乐死组相比，直到损伤（M/E 损伤）的中等强度训练（M）在受伤后 7 天导致更好的伤口闭合（P<0.05），并且两个中等强度组的再上皮化率均高于对照组（M/E 损伤=85.9%，M/E 安乐死=96.4%和 M/CG=79.9%；P<0.05）。用苏木精-伊红、苦味酸-天狼星红和 α-SMA 染色的 M/E 损伤和 M/E 安乐死组的切片显示了所有训练组和对照组中最成熟的肉芽组织。因此，中等强度的身体训练可改善皮肤伤口愈合。
