a State Key Laboratory of Stem Cell and Reproductive Biology , Institute of Zoology, Chinese Academy of Sciences , Beijing , P.R. China.
b University of Chinese Academy of Sciences , Beijing , P.R. China.
Nucleus. 2017 Sep 3;8(5):461-468. doi: 10.1080/19491034.2017.1330237. Epub 2017 Jun 19.
RNF20/Bre1 mediated H2B ubiquitination (H2Bub) has various physiologic functions. Recently, we found that H2Bub participates in meiotic recombination by promoting chromatin relaxation during meiosis. We then analyzed the phylogenetic relationships among the E3 ligase for H2Bub, its E2 Rad6 and their partner WW domain-containing adaptor with a coiled-coil (WAC) or Lge1, and found that the molecular mechanism underlying H2Bub is evolutionarily conserved from yeast to mammals. However, RNF20 has diverse physiologic functions in different organisms, which might be caused by the evolutionary divergency of their domain/motif architectures. In the current extra view, we not only elucidate the evolutionarily conserved molecular mechanism underlying H2Bub, but also discuss the diverse physiologic functions of RNF20 during meiosis.
RNF20/Bre1 介导的 H2B 泛素化（H2Bub）具有多种生理功能。最近，我们发现 H2Bub 通过在减数分裂过程中促进染色质松弛参与减数分裂重组。然后，我们分析了 H2Bub 的 E3 连接酶、其 E2 Rad6 及其伴侣卷曲螺旋（WAC）或 Lge1 之间的系统发育关系，发现从酵母到哺乳动物，H2Bub 的分子机制在进化上是保守的。然而，RNF20 在不同的生物体中有不同的生理功能，这可能是由于它们的结构域/基序结构的进化分歧造成的。在本期增刊中，我们不仅阐明了 H2Bub 背后保守的分子机制，还讨论了 RNF20 在减数分裂过程中的多种生理功能。
