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RNF20 通过招募 TPM3 到着丝粒和纺锤体两极来调节卵母细胞减数分裂纺锤体的组装。

RNF20 Regulates Oocyte Meiotic Spindle Assembly by Recruiting TPM3 to Centromeres and Spindle Poles.

机构信息

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, China.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing, 100101, China.

出版信息

Adv Sci (Weinh). 2024 Apr;11(13):e2306986. doi: 10.1002/advs.202306986. Epub 2024 Jan 19.

DOI:10.1002/advs.202306986
PMID:38240347
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10987117/
Abstract

Previously a ring finger protein 20 (RNF20) is found to be essential for meiotic recombination and mediates H2B ubiquitination during spermatogenesis. However, its role in meiotic division is still unknown. Here, it is shown that RNF20 is localized at both centromeres and spindle poles, and it is required for oocyte acentrosomal spindle organization and female fertility. RNF20-depleted oocytes exhibit severely abnormal spindle and chromosome misalignment caused by defective bipolar organization. Notably, it is found that the function of RNF20 in spindle assembly is not dependent on its E3 ligase activity. Instead, RNF20 regulates spindle assembly by recruiting tropomyosin3 (TPM3) to both centromeres and spindle poles with its coiled-coil motif. The RNF20-TPM3 interaction is essential for acentrosomal meiotic spindle assembly. Together, the studies uncover a novel function for RNF20 in mediating TPM3 recruitment to both centromeres and spindle poles during oocyte spindle assembly.

摘要

先前的研究发现，环指蛋白 20（RNF20）在减数分裂重组中是必需的，并在精子发生过程中介导 H2B 的泛素化。然而，其在减数分裂中的作用尚不清楚。本研究表明，RNF20 定位于着丝粒和纺锤体两极，对于卵母细胞无中心体纺锤体的组织和雌性生育能力是必需的。RNF20 耗竭的卵母细胞表现出严重的纺锤体异常和染色体错位，这是由于双极组织缺陷所致。值得注意的是，研究发现 RNF20 在纺锤体组装中的功能不依赖于其 E3 连接酶活性。相反，RNF20 通过其卷曲螺旋结构域将原肌球蛋白 3（TPM3）募集到着丝粒和纺锤体两极，从而调节纺锤体的组装。RNF20-TPM3 相互作用对于无中心体的减数分裂纺锤体组装是必需的。总之，这些研究揭示了 RNF20 在介导 TPM3 招募到卵母细胞纺锤体组装过程中的着丝粒和纺锤体两极的新功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/eb29e541f13c/ADVS-11-2306986-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/1e294aa669f1/ADVS-11-2306986-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/630418ea746a/ADVS-11-2306986-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/1a79580e1a05/ADVS-11-2306986-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/353a85242672/ADVS-11-2306986-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/ce64849ed0b6/ADVS-11-2306986-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/ce3867115884/ADVS-11-2306986-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/45784ef8f9fc/ADVS-11-2306986-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/eb29e541f13c/ADVS-11-2306986-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/1e294aa669f1/ADVS-11-2306986-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/630418ea746a/ADVS-11-2306986-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/1a79580e1a05/ADVS-11-2306986-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/353a85242672/ADVS-11-2306986-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/ce64849ed0b6/ADVS-11-2306986-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/ce3867115884/ADVS-11-2306986-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/45784ef8f9fc/ADVS-11-2306986-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a53b/10987117/eb29e541f13c/ADVS-11-2306986-g002.jpg

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