National Development Institute of Korean Medicine, Jangheung, 59338, Republic of Korea.
Department of Food and Nutrition, Sunchon National University, Suncheon, 57922, Republic of Korea.
Biomed Pharmacother. 2017 Sep;93:165-171. doi: 10.1016/j.biopha.2017.06.037. Epub 2017 Jun 17.
This study investigated the effects young leaves of reed (Phragmites communis) water extract (YLR) on melanogenesis and oxidative stress using B16F10 cells. YLR decreased the intracellular melanin content, protein expression and enzyme activity of tyrosinase in a dose-dependent manner. YLR significantly decreased the gene and protein expression of melanogeneis-related proteins, such as microphthalmia-associated transcription factor (MITF), and tyrosinase-related protein-1 and -2. In addition, YLR up-regulated the melanogenesis inhibitory proteins, extracellular signal-regulated kinase (ERK) and protein kinase B (AKT), while it dose-dependently down-regulated p38 and cAMP response element-binding protein (CREB). Moreover, YLR significantly reduced HO-induced reactive oxygen species levels in B16F10 cells and showed antioxidant activity based on DPPH and ABTS free radical scavenging activity and SOD-like activity. These results suggest that YLR have anti-melanogensis properties that function through regulation of the CREB/MITF/tyrosinase pathway in B16F10 cells and antioxidant activity. Overall, these findings indicate that YLR has the potential for use in treatment of skin disorders and skin-whitening.
本研究采用 B16F10 细胞，探讨了芦竹幼叶水提物（YLR）对黑色素生成和氧化应激的影响。结果表明，YLR 呈剂量依赖性降低细胞内黑色素含量、酪氨酸酶蛋白表达和酶活性。YLR 显著下调黑色素生成相关蛋白微phthalmia 相关转录因子（MITF）、酪氨酸酶相关蛋白-1 和 -2 的基因和蛋白表达。此外，YLR 上调黑色素生成抑制蛋白细胞外信号调节激酶（ERK）和蛋白激酶 B（AKT），同时呈剂量依赖性地下调 p38 和 cAMP 反应元件结合蛋白（CREB）。此外，YLR 可显著降低 B16F10 细胞中原代培养的 HO 诱导的活性氧水平，并基于 DPPH 和 ABTS 自由基清除活性和 SOD 样活性表现出抗氧化活性。这些结果表明，YLR 具有抗黑色素生成特性，可通过调节 B16F10 细胞中的 CREB/MITF/tyrosinase 通路和抗氧化活性发挥作用。总之，这些发现表明 YLR 具有治疗皮肤疾病和美白的潜力。
