College of Geography and Environment, Shandong Normal University, 88 Wenhua Donglu, Jinan 250014, Shandong, PR China; College of Life Science, Shandong Normal University, 88 Wenhua Donglu, Jinan 250014, Shandong, PR China; Institute of Environment and Ecology, Shandong Normal University, 88 Wenhua Donglu, Jinan 250014, Shandong, PR China.
College of Geography and Environment, Shandong Normal University, 88 Wenhua Donglu, Jinan 250014, Shandong, PR China; College of Life Science, Shandong Normal University, 88 Wenhua Donglu, Jinan 250014, Shandong, PR China.
Bioresour Technol. 2017 Oct;241:849-856. doi: 10.1016/j.biortech.2017.06.019. Epub 2017 Jun 15.
An activated sludge sequencing batch reactor (SBR) was fed with synthetic wastewater containing ofloxacin (OFL) for 52days to study the overall performance of the SBR, the characteristics of extracellular polymeric substances (EPS) and the bacterial community shift. Removal efficiencies of chemical oxygen demand, ammonia nitrogen, phosphorus and OFL were maintained at 90%, 96%, 80% and 65%, respectively. The EPS contents increased with increasing OFL concentration because more EPS was secreted to protect cells from OFL damage. Moreover, the EPS compositions shifted. For denitrifying bacteria, the read number of Pseudomonas and Bacillus sharply decreased initially (p<0.05) and increased from Day 25 to Day 50, which agreed with the tendency of Nitrosomonadaceae (ammonia oxidizer), while Paracoccus significantly decreased (p<0.05). The read number of Rhodocyclaceae, a phosphorus-accumulating bacterium, increased. Other functional microbes such as Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) sharply decreased under OFL pressure (p<0.05).
采用人工合成废水，在序批式活性污泥法（SBR）中连续运行 52 天，研究了系统对含氟沙星（OFL）废水的整体处理效能、胞外聚合物（EPS）特性变化及微生物群落结构演替。结果表明，COD、氨氮、磷和 OFL 的去除率分别维持在 90%、96%、80%和 65%。随着 OFL 浓度的增加，EPS 含量逐渐增加，因为更多的 EPS 被分泌出来以保护细胞免受 OFL 损伤。此外，EPS 组成发生了变化。对于反硝化菌，假单胞菌和芽孢杆菌的相对丰度起初（p<0.05）急剧下降，从第 25 天到第 50 天逐渐增加，与氨氧化菌（Nitrosomonadaceae）的趋势一致，而过氧化氢酶（Paracoccus）的相对丰度显著下降（p<0.05）。聚磷菌（Rhodocyclaceae）的相对丰度增加。在 OFL 压力下，其他功能微生物（如亚硝酸盐氧化菌（Nitrospirales）和厌氧氨氧化菌（Planctomyces））的相对丰度急剧下降（p<0.05）。
