School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, China.
Environ Sci Pollut Res Int. 2018 Apr;25(10):9342-9350. doi: 10.1007/s11356-018-1221-1. Epub 2018 Jan 18.
Since extensive application, an increasing amount of antibiotics has been released into wastewater treatment plants. In this study, the enhanced biological phosphorus removal (EBPR) system was fed with synthetic wastewater containing erythromycin (ERY) and oxytetracycline (OTC) for 7 days to evaluate the variations of solution ortho-P (SOP), volatile fatty acid (VFA), poly-bhydroxyalkanoates (PHAs), specific oxygen uptake rater (SOUR), and microbial community in the EBPR system. The obtained results showed that the P-removal efficiency decreased to 0.0%, and at the end of the experiment, only less than 20% of the VFA could be consumed. Besides, the variable processes of P and PHAs were destroyed. Moreover, to better grasp the inhibitory mechanism of antibiotics, microbial community compositions of activated sludge sampled in all reactors were investigated by high-throughput sequencing techniques. Results of comparative and evolutionary analysis revealed that high concentrations (5 and 10 mg/L) of ERY and OTC could seriously shift microbial communities, while combined antibiotics could induce more. Additionally, Accumulibacter and Competibacter were two primary microorganisms at the genus level in the EBPR system. Accumulibacter decreased seriously for exposure to antibiotics, while Competibacter increased in all experimental reactors especially in combined antibiotics reactor.
自广泛应用以来，越来越多的抗生素被排放到污水处理厂。在这项研究中，采用含有红霉素（ERY）和土霉素（OTC）的合成废水对强化生物除磷（EBPR）系统进行了 7 天的喂养，以评估 EBPR 系统中溶液正磷酸盐（SOP）、挥发性脂肪酸（VFA）、聚羟基烷酸酯（PHAs）、比需氧量（SOUR）和微生物群落的变化。结果表明，磷去除效率降至 0.0%，实验结束时，只有不到 20%的 VFA 可以被消耗。此外，磷和 PHAs 的可变过程被破坏。此外，为了更好地掌握抗生素的抑制机制，采用高通量测序技术对所有反应器中采集的活性污泥的微生物群落组成进行了研究。比较和进化分析的结果表明，高浓度（5 和 10mg/L）的 ERY 和 OTC 可严重改变微生物群落，而联合抗生素则可诱导更多的变化。此外，在 EBPR 系统中，Accumulibacter 和 Competibacter 是两个主要的属水平微生物。抗生素暴露使 Accumulibacter 严重减少，而 Competibacter 在所有实验反应器中增加，尤其是在联合抗生素反应器中增加。
