College of Chemistry, Chemical Engineering and Biotechnology, Donghua University,Shanghai 201620, China.
UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:245-254. doi: 10.1016/j.msec.2017.04.058. Epub 2017 May 5.
In this work, we aimed to develop new materials to reduce the secondary injuries which can be imparted when replacing wound dressings. Electrospun fibers based on the thermoresponsive polymer poly(N-isopropylacrylamide) (PNIPAAm), poly(l-lactic acid-co-ɛ-caprolactone) (PLCL), and the antibiotic ciprofloxacin (CIF) were prepared. The water contact angle of fibers made from a blend of PNIPAAm and PLCL changed dramatically when the temperature was increased above 32°C. Sustained release of CIF from the formulations was observed over >200h. Moreover, L929 fibroblasts could proliferate on the fibers, indicating their biocompatibility. The CIF-loaded fibers were found to have potent antibacterial activity against E. coli and S. aureus. In vivo tests on rats indicated that CIF-loaded thermosensitive fibers have enhanced healing performance compared to CIF-loaded PLCL fibers or a commercial gauze. Electrospun PNIPAAm/PLCL fibers loaded with CIF thus have great promise in the development of new wound dressing materials.
在这项工作中，我们旨在开发新材料，以减少更换伤口敷料时可能造成的二次损伤。我们制备了基于温敏聚合物聚（N-异丙基丙烯酰胺）（PNIPAAm）、聚（L-乳酸-co-ε-己内酯）（PLCL）和抗生素环丙沙星（CIF）的电纺纤维。当温度升高到 32°C 以上时，由 PNIPAAm 和 PLCL 共混物制成的纤维的水接触角会发生剧烈变化。在 >200h 内观察到 CIF 从配方中的持续释放。此外，L929 成纤维细胞可以在纤维上增殖，表明其生物相容性。载有 CIF 的纤维对大肠杆菌和金黄色葡萄球菌具有很强的抗菌活性。大鼠体内试验表明，与载有 CIF 的 PLCL 纤维或商业纱布相比，载有 CIF 的热敏纤维具有更好的愈合性能。因此，负载 CIF 的电纺 PNIPAAm/PLCL 纤维在开发新型伤口敷料材料方面具有很大的应用前景。
