S Sonia, H Linda Jeeva Kumari, K Ruckmani, M Sivakumar
Division of Nanoscience and Technology, Bharathidasan Institute of Technology, Anna University, Tiruchirappalli 620024, Tamil Nadu, India.
National Facility for Drug Development (NFDD) for Academia, Pharmaceutical and Allied Industries, Bharathidasan Institute of Technology, Anna University, Tiruchirappalli 620024, Tamil Nadu, India; Department of Pharmaceutical Technology, Centre for Excellence in Nanobio Translational Research (CENTRE), Bharathidasan Institute of Technology, Anna University, Tiruchirappalli 620024, Tamil Nadu, India.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:581-589. doi: 10.1016/j.msec.2017.05.059. Epub 2017 May 10.
Nanocosmeceuticals are promising applications of nanotechnology in personal care industries. Zinc oxide is an inorganic material that is non-toxic and skin compatible with self-cleansing and microbicidal properties. Herein, exploitation of colloidal zinc oxide nanoparticles (ZnONps) as potent biomaterial for a topical formulation of cosmetic and dermatological significance is employed. ZnONps were green synthesized using environmentally benign Adhatoda vasica leaf extract and characterized by UV-Vis absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), dynamic light scattering (DLS), high-resolution transmission electron microscopy (HR-TEM) and energy-dispersive X-ray spectroscopy (EDX). The results reveal that the biosynthesized ZnONps exhibit an absorption peak at 352nm. XRD and HR-TEM analyses confirm the hexagonal wurtzite structure of ZnONps with particle size of about 10nm to 12nm. Elemental analysis by EDX confirms the presence of zinc and oxygen. Zeta potential of -24.6mV affirms the stability of nanoparticles. The antibacterial and antifungal activities of biosynthesized ZnONps exhibit mean zone of inhibition from 08.667±0.282 to 21.666±0.447 (mm) and 09.000±0.177 to 19.000±0.307 (mm) respectively, in a dose-dependent manner. The IC value exerted from the antioxidant activity of ZnONps is found to be 139.27μgmL. ZnONps infused cold cream formulation of microbicidal and antioxidant properties was further tested against clinical skin pathogens. The nano-based cold cream exhibited significant inhibitory action against Candida sp., which showed resistance against a commercial antifungal cream (2%). Therefore, this study demonstrates the exploitation of ZnONps as promising colloidal drug carriers in cosmeceuticals that can significantly alleviate human skin infections and oxidative stress induced cellular damage.
纳米化妆品是纳米技术在个人护理行业中颇具前景的应用领域。氧化锌是一种无机材料，无毒且与皮肤相容，具有自清洁和杀菌特性。在此，我们利用胶体氧化锌纳米颗粒（ZnONps）作为具有重要美容和皮肤病学意义的局部制剂的有效生物材料。使用环境友好的鸭嘴花叶片提取物对ZnONps进行绿色合成，并通过紫外可见吸收光谱、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、动态光散射（DLS）、高分辨率透射电子显微镜（HR-TEM）和能量色散X射线光谱（EDX）对其进行表征。结果表明，生物合成的ZnONps在352nm处有一个吸收峰。XRD和HR-TEM分析证实了ZnONps的六方纤锌矿结构，粒径约为10nm至12nm。EDX的元素分析证实了锌和氧的存在。-24.6mV的zeta电位证实了纳米颗粒的稳定性。生物合成的ZnONps的抗菌和抗真菌活性分别以剂量依赖的方式表现出平均抑菌圈为08.667±0.282至21.666±0.447（mm）和09.000±0.177至19.000±0.307（mm）。发现ZnONps抗氧化活性的IC值为139.27μg/mL。对具有杀菌和抗氧化特性的含ZnONps的冷霜制剂进一步针对临床皮肤病原体进行了测试。基于纳米的冷霜对念珠菌属表现出显著的抑制作用，而念珠菌属对市售抗真菌霜（2%）具有抗性。因此，本研究证明了ZnONps作为化妆品中颇具前景的胶体药物载体的应用，其可显著减轻人类皮肤感染和氧化应激诱导的细胞损伤。
