Department of Botany, Bacha Khan University, Charsadda, Pakistan.
Department of Biology, The University of Haripur, Haripur, Pakistan.
Microsc Res Tech. 2022 Jun;85(6):2338-2350. doi: 10.1002/jemt.24090. Epub 2022 Mar 16.
In the recent years, green synthesis of zinc oxide nanoparticles (ZnONPs) using plant extracts and phytochemicals has gained significant attention. In present research study, facile, green, and tunable ZnONPs were biosynthesized from Rhamnella gilgitica leaf aqueous extract as a strong reducing and stabilizing agents. The prepared ZnONPs@Rhamnella were characterized and validated using common nanotechnology techniques (UV-Vis, XRD, EDX, FT-IR, SEM, TEM, DLS, and Raman) and revealed spherical morphology with particle size ~21 nm. The asynthesized ZnONPs were further evaluated for different biological applications. Strong antimicrobial efficacies were reported for ZnONPs using disc-diffusion method and were capable of rendering significant antimicrobial potential. ZnONPs were evaluated against HepG2 (IC : 18.40 μg/ml) and HUH7 (IC : 20.59 μg/ml) cancer cell lines and revealed strong anticancer properties. Dose-dependent MTT cytotoxicity assay was confirmed using Leishmania tropica "KWH23 strain" (promastigote: IC : 26.78 μg/ml and amastigote: IC : 29.57 μg/ml). Antioxidant activities (DPPH: 93.36%, TAC: 72.43%) were performed to evaluate their antioxidant potentials. Further, protein kinase and α-amylase inhibition assays were determined. Biocompatibility assays were done using human RBCs and macrophages thus revealed biosafe and non-toxic nature of ZnONPs@Rhamnella. In current experiment, we concluded that greenly orchestrated ZnONPs is an attractive, non-toxic and ecofriendly candidate and showed potential biological activities. In future, different clinical trials and in vivo studies are necessary for the confirmation of these remedial properties of ZnONPs using different animal models. RESEARCH HIGHLIGHTS: Greenly orchestrated ZnONPs were synthesized using Rhamnella gilgitica leaves broth. Analytical techniques such as UV, SEM, TEM, XRD, FTIR, DLS, and Raman confirmed synthesis of ZnONPs. Green ZnONPs determined strong antimicrobial, cytotoxic, and antioxidant potentials. Significant enzyme inhibition and biocompatibility assays were investigated.
近年来，利用植物提取物和植物化学物质进行氧化锌纳米粒子（ZnONPs）的绿色合成已经引起了广泛关注。在本研究中，我们使用吉尔吉特拉汉姆菌（Rhamnella gilgitica）叶的水提物作为强还原剂和稳定剂，简便、绿色、可调的 ZnONPs 被生物合成。所制备的 ZnONPs@Rhamnella 通过常见的纳米技术技术（UV-Vis、XRD、EDX、FT-IR、SEM、TEM、DLS 和 Raman）进行了表征和验证，显示出约 21nm 的球形形态和颗粒尺寸。进一步评估了合成的 ZnONPs 在不同生物应用中的性能。使用圆盘扩散法报告了 ZnONPs 的强抗菌功效，并且能够表现出显著的抗菌潜力。评估了 ZnONPs 对 HepG2（IC：18.40μg/ml）和 HUH7（IC：20.59μg/ml）癌细胞系的抗菌作用，显示出较强的抗癌特性。使用利什曼原虫“KWH23 株”（前鞭毛体：IC：26.78μg/ml 和无鞭毛体：IC：29.57μg/ml）进行了依赖剂量的 MTT 细胞毒性测定，证实了剂量依赖性细胞毒性。进行了抗氧化活性（DPPH：93.36%，TAC：72.43%）以评估其抗氧化潜力。进一步进行了蛋白激酶和α-淀粉酶抑制测定。使用人 RBC 和巨噬细胞进行了生物相容性测定，从而显示出 ZnONPs@Rhamnella 的生物相容性和非毒性。在目前的实验中，我们得出结论，绿色协调的 ZnONPs 是一种有吸引力的、无毒的和环保的候选物，并显示出潜在的生物活性。在未来，需要使用不同的动物模型进行不同的临床试验和体内研究，以确认 ZnONPs 的这些治疗特性。研究亮点： 使用吉尔吉特拉汉姆菌（Rhamnella gilgitica）叶肉汤合成了绿色协调的 ZnONPs。使用 UV、SEM、TEM、XRD、FTIR、DLS 和 Raman 等分析技术证实了 ZnONPs 的合成。绿色 ZnONPs 表现出强抗菌、细胞毒性和抗氧化能力。进行了显著的酶抑制和生物相容性测定。
