Departamento de Ingenierías Química, Electrónica y Biomédica, DCI, Universidad de Guanajuato, Loma del Bosque 103, 37150 León, Gto., Mexico; Departamento de Química, DCNE, Universidad de Guanajuato, Noria alta s/n, 36050 Guanajuato, Gto., Mexico; Ingeniería en Biotecnología, Universidad Politécnica de Pénjamo, Carretera Irapuato-La Piedad Km 44, 36921, Pénjamo, Gto., Mexico.
Departamento de Ingenierías Química, Electrónica y Biomédica, DCI, Universidad de Guanajuato, Loma del Bosque 103, 37150 León, Gto., Mexico; Departamento de Química, DCNE, Universidad de Guanajuato, Noria alta s/n, 36050 Guanajuato, Gto., Mexico.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:793-801. doi: 10.1016/j.msec.2017.05.118. Epub 2017 May 18.
In this work, hydrolysates of extracellular matrix (hECM) were obtained from rat tail tendon (TR), bovine Achilles tendon (TAB), porcine small intestinal submucosa (SIS) and bovine pericardium (PB), and they were polymerized to generate ECM hydrogels. The composition of hECM was evaluated by quantifying the content of sulphated glycosaminoglycans (sGAG), fibronectin and laminin. The polymerization process, structure, physicochemical properties, in vitro degradation and biocompatibility were studied and related to their composition. The results indicated that the hECM derived from SIS and PB were significantly richer in sGAG, fibronectin and laminin, than those derived from TAB and TR. These differences in hECM composition influenced the polymerization and the structural characteristics of the fibrillar gel network. Consequently, the swelling, mechanics and degradation of the hydrogels showed a direct relationship with the remaining composition. Moreover, the cytocompatibility and the secretion of transforming growth factor beta-1 (TGF-β1) by macrophages were enhanced in hydrogels with the highest residual content of ECM biomolecules. The results of this work evidenced the role of the ECM molecules remaining after both decellularization and hydrolysis steps to produce tissue derived hydrogels with structure and properties tailored to enhance their performance in tissue engineering and regenerative medicine applications.
在这项工作中，从大鼠尾腱（TR）、牛跟腱（TAB）、猪小肠黏膜下层（SIS）和牛心包（PB）中获得细胞外基质（hECM）的水解产物，并将其聚合以生成 ECM 水凝胶。通过定量测定硫酸化糖胺聚糖（sGAG）、纤连蛋白和层粘连蛋白的含量来评估 hECM 的组成。研究了聚合过程、结构、物理化学性质、体外降解和生物相容性，并将其与组成相关联。结果表明，SIS 和 PB 来源的 hECM 在 sGAG、纤连蛋白和层粘连蛋白方面明显比 TAB 和 TR 来源的丰富。hECM 组成的这些差异影响聚合和纤维状凝胶网络的结构特征。因此，水凝胶的溶胀、力学和降解与剩余的组成直接相关。此外，具有最高 ECM 生物分子残留含量的水凝胶增强了巨噬细胞的细胞相容性和转化生长因子β-1（TGF-β1）的分泌。这项工作的结果证明了细胞外基质分子在脱细胞和水解步骤后残留的作用，以产生结构和性能适合增强其在组织工程和再生医学应用中的性能的组织衍生水凝胶。
