Caldera-Villalobos Martín, Claudio-Rizo Jesús A, Cabrera-Munguía Denis A
Facultad de Ciencias Químicas. Universidad Autónoma de Coahuila. Unidad Saltillo. Saltillo, Coahuila, 25280, México.
ADMET DMPK. 2024 Mar 5;12(2):359-377. doi: 10.5599/admet.2074. eCollection 2024.
In this study, we examined the impact of Zn-bioMOF structures on the physical and chemical characteristics as well as the biocompatibility of a matrix composed of semi-interpenetrating polymeric networks (semi-IPN) made from collagen and L-tyrosine-based polyelectrolytes.
We hydrothermally synthesized L-1, ZIF-8H Zn-bioMOFs, and the Zn-(L-His) complex, utilizing L-histidine, a bioactive amino acid, as a ligand. These metal-organic compounds primarily enhance the mechanical properties of the novel composite hydrogels through physical interactions such as hydrogen bonds and dipolar interactions. They also accelerate the gelation process. Composites containing Zn-bioMOFs exhibited greater biocompatibility than the collagen/polyelectrolyte matrix alone, as evidenced by cytotoxicity assays conducted with porcine fibroblasts, human monocytes, and RAW 264.7 cells. Furthermore, the evaluated materials did not exhibit hemolysis. We investigated the influence of Zn-bioMOFs on cell signaling by measuring the levels of crucial cytokines involved in the healing process, such as MCP-1, TGF-β, IL-10, and TNF-α secreted by human monocytes.
The composite with Zn(L-His)2 promoted the secretion of MCP-1, TGF-β, and IL-10, while a decrease in TNF-α secretion was observed with the composite containing ZIF-8H. Zn-bioMOFs enhanced certain aspects of the biomedical and physicochemical properties of the composite hydrogels.
Although the overall performance of the tested materials did not differ significantly, it is worth noting that the presence of Zn-bioMOFs in biopolymeric hydrogels modulated the metabolic activity of cells important for healing and their cytokine signaling, leading to improved biomedical performance.
在本研究中，我们考察了锌基生物金属有机框架结构对由胶原蛋白和基于L-酪氨酸的聚电解质制成的半互穿聚合物网络（半互穿网络聚合物，semi-IPN）组成的基质的物理化学特性以及生物相容性的影响。
我们以具有生物活性的氨基酸L-组氨酸作为配体，通过水热法合成了L-1、ZIF-8H锌基生物金属有机框架材料以及Zn-(L-His)配合物。这些金属有机化合物主要通过氢键和偶极相互作用等物理相互作用增强新型复合水凝胶的机械性能。它们还加速了凝胶化过程。用猪成纤维细胞、人单核细胞和RAW 264.7细胞进行的细胞毒性试验表明，含锌基生物金属有机框架材料的复合材料比单独的胶原蛋白/聚电解质基质表现出更高的生物相容性。此外，所评估的材料未表现出溶血现象。我们通过测量人单核细胞分泌的参与愈合过程的关键细胞因子（如MCP-1、TGF-β、IL-10和TNF-α）的水平，研究了锌基生物金属有机框架材料对细胞信号传导的影响。
含Zn(L-His)2的复合材料促进了MCP-1、TGF-β和IL-10的分泌，而含ZIF-8H的复合材料则使TNF-α的分泌减少。锌基生物金属有机框架材料增强了复合水凝胶在生物医学和物理化学性能方面的某些特性。
尽管测试材料的整体性能没有显著差异，但值得注意的是，生物聚合物水凝胶中锌基生物金属有机框架材料的存在调节了对愈合重要的细胞的代谢活性及其细胞因子信号传导，从而改善了生物医学性能。
