Yamaguchi N, Sugimoto M, Kawai K
Clin Chim Acta. 1985 Jun 30;149(1):47-54. doi: 10.1016/0009-8981(85)90272-4.
Two different types of gamma-glutamyltranspeptidase (gamma-GTP) have been found in normal human pancreas following bromelain treatment. On the other hand, three human pancreatic ductal cell carcinomas have only a single type of gamma-GTP upon analysis with polyacrylamide gel electrophoresis, anion-exchange column chromatography and isoelectric focusing. Carcinoma gamma-GTPs were almost identical to one of the two types of normal pancreatic gamma-GTPs. The gamma-GTP from pancreatic carcinomas bound to anion-exchange column and was eluted at the same NaCl fractions as normal pancreatic gamma-GTP. The properties of pancreatic carcinoma-gamma-GTP, as assessed by binding to concanavalin A and lentil lectin affinity columns, were also similar to one of the two enzymes of normal pancreas. No apparent difference in isoelectric points was found between the carcinoma gamma-GTPs and one of the two normal pancreatic gamma-GTPs.
在经菠萝蛋白酶处理的正常人胰腺中发现了两种不同类型的γ-谷氨酰转肽酶（γ-GTP）。另一方面，通过聚丙烯酰胺凝胶电泳、阴离子交换柱色谱法和等电聚焦分析，三种人胰腺导管细胞癌仅有一种γ-GTP。癌组织中的γ-GTP与正常胰腺两种γ-GTP中的一种几乎相同。胰腺癌的γ-GTP与阴离子交换柱结合，并在与正常胰腺γ-GTP相同的氯化钠组分中洗脱。通过与伴刀豆球蛋白A和扁豆凝集素亲和柱结合评估，胰腺癌γ-GTP的性质也与正常胰腺的两种酶之一相似。癌组织γ-GTP与正常胰腺两种γ-GTP之一在等电点上没有明显差异。
