Yamaguchi N, Kawai K, Ashihara T
Clin Chim Acta. 1986 Jan 30;154(2):133-40. doi: 10.1016/0009-8981(86)90005-7.
Two types of gamma-glutamyltranspeptidase (gamma-GTP) were identified in normal human pancreas, but ductal cell carcinomas of the pancreas showed only one type on analysis with polyacrylamide gel electrophoresis, anion exchange column chromatography, concanavalin A affinity chromatography and isoelectric focusing. gamma-GTP from carcinoma tissue was almost identical to the sialic acid-rich type of normal pancreatic gamma-GTP, and our methods did not evidence a difference between the two. However, E-PHA lectin column chromatography demonstrated differences in their elution pattern. Compared with normal pancreatic gamma-GTPs, the elution pattern of the carcinoma gamma-GTP was slowly delayed by the E-PHA lectin column. This finding suggests the presence of a bisecting N-acetylglucosamine-rich sugar chain in pancreatic carcinoma gamma-GTP, as well as the possible presence of a different sugar structure in the carcinoma gamma-GTP.
在正常人体胰腺中鉴定出两种类型的γ-谷氨酰转肽酶（γ-GTP），但通过聚丙烯酰胺凝胶电泳、阴离子交换柱色谱、伴刀豆球蛋白A亲和色谱和等电聚焦分析发现，胰腺导管细胞癌仅显示一种类型。癌组织中的γ-GTP与正常胰腺富含唾液酸类型的γ-GTP几乎相同，并且我们的方法未证明两者之间存在差异。然而，E-PHA凝集素柱色谱显示它们的洗脱模式存在差异。与正常胰腺γ-GTP相比，癌γ-GTP在E-PHA凝集素柱上的洗脱模式延迟缓慢。这一发现表明胰腺癌γ-GTP中存在富含平分型N-乙酰葡糖胺的糖链，以及癌γ-GTP中可能存在不同的糖结构。
