蛋白质和脂多糖插入细菌外膜。
Insertion of proteins and lipopolysaccharide into the bacterial outer membrane.
作者信息
Botos Istvan, Noinaj Nicholas, Buchanan Susan K
机构信息
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Markey Center for Structural Biology, Department of Biological Sciences, and the Purdue Institute for Inflammation, Immunology and Infectious Diseases, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Philos Trans R Soc Lond B Biol Sci. 2017 Aug 5;372(1726). doi: 10.1098/rstb.2016.0224.
The bacterial outer membrane contains phospholipids in the inner leaflet and lipopolysaccharide (LPS) in the outer leaflet. Both proteins and LPS must be frequently inserted into the outer membrane to preserve its integrity. The protein complex that inserts LPS into the outer membrane is called LptDE, and consists of an integral membrane protein, LptD, with a separate globular lipoprotein, LptE, inserted in the barrel lumen. The protein complex that inserts newly synthesized outer-membrane proteins (OMPs) into the outer membrane is called the BAM complex, and consists of an integral membrane protein, BamA, plus four lipoproteins, BamB, C, D and E. Recent structural and functional analyses illustrate how these two complexes insert their substrates into the outer membrane by distorting the membrane component (BamA or LptD) to directly access the lipid bilayer.This article is part of the themed issue 'Membrane pores: from structure and assembly, to medicine and technology'.
细菌外膜的内膜层含有磷脂，外膜层含有脂多糖（LPS）。蛋白质和LPS都必须频繁插入外膜以维持其完整性。将LPS插入外膜的蛋白质复合物称为LptDE，它由一个整合膜蛋白LptD和一个插入桶状内腔的单独球状脂蛋白LptE组成。将新合成的外膜蛋白（OMP）插入外膜的蛋白质复合物称为BAM复合物，它由一个整合膜蛋白BamA加上四个脂蛋白BamB、C、D和E组成。最近的结构和功能分析表明，这两种复合物如何通过扭曲膜成分（BamA或LptD）直接进入脂质双层，从而将其底物插入外膜。本文是主题为“膜孔：从结构与组装到医学与技术”的一部分。
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