Sengel Jason T, Wallace Mark I
Department of Chemistry, Chemistry Research Laboratory, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
Department of Chemistry, King's College London, Britannia House, 7 Trinity Street, London, SE1 1DB, UK
Philos Trans R Soc Lond B Biol Sci. 2017 Aug 5;372(1726). doi: 10.1098/rstb.2016.0227.
Electroporation is a common tool for gene transfection, tumour ablation, sterilization and drug delivery. Using experimental methods, we explore the temperature dependence of electropore formation in a model membrane system (droplet-interface bilayers), using optical single-channel recording to image the real-time gating of individual electropores. We investigate the influence of the agarose substrate on electropores formed in this system. Furthermore, by examining the temperature-dependent kinetics of pore opening and closure we are able to estimate a barrier to pore opening in 1,2-diphytanoyl--glycero-3-phosphocholine (DPhPC) membranes to be 25.0 ± 8.3 , in agreement with previous predictions. Overall these measurements help support the toroidal model of membrane electroporation.This article is part of the themed issue 'Membrane pores: from structure and assembly, to medicine and technology'.
电穿孔是基因转染、肿瘤消融、杀菌和药物递送的常用工具。我们采用实验方法，利用光学单通道记录对单个电穿孔的实时门控进行成像，研究了模型膜系统（液滴界面双层膜）中电穿孔形成的温度依赖性。我们考察了琼脂糖底物对该系统中形成的电穿孔的影响。此外，通过研究孔开放和关闭的温度依赖性动力学，我们能够估计1,2 - 二植酰 - sn -甘油 - 3 - 磷酸胆碱（DPhPC）膜中孔开放的能垒为25.0 ± 8.3 ，这与先前的预测一致。总体而言，这些测量结果有助于支持膜电穿孔的环形模型。本文是主题为“膜孔：从结构与组装到医学与技术”特刊的一部分。
