抗生素耐药性作为一种应激反应：通过诱导严格应激反应恢复耐甲氧西林金黄色葡萄球菌“辅助”（）突变体中的高水平苯唑西林耐药性
Antibiotic Resistance as a Stress Response: Recovery of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus "Auxiliary" () Mutants by Induction of the Stringent Stress Response.
作者信息
Kim Choon Keun, Milheiriço Catarina, de Lencastre Hermínia, Tomasz Alexander
机构信息
Laboratory of Microbiology & Infectious Diseases, The Rockefeller University, New York, New York, USA.
Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica (ITQB) da Universidade Nova de Lisboa, Oeiras, Portugal.
出版信息
Antimicrob Agents Chemother. 2017 Jul 25;61(8). doi: 10.1128/AAC.00313-17. Print 2017 Aug.
Abstract
Studies with methicillin-resistant (MRSA) strain COL have shown that the optimal resistance phenotype requires not only but also a large number of "auxiliary genes" identified by Tn mutagenesis. The majority of auxiliary mutants showed greatly increased levels of oxacillin resistance when grown in the presence of sub-MICs of mupirocin, suggesting that the mechanism of reduced resistance in the auxiliary mutants involved the interruption of a stringent stress response, causing reduced production of penicillin-binding protein 2A (PBP 2A).
摘要
对耐甲氧西林金黄色葡萄球菌（MRSA）菌株COL的研究表明，最佳耐药表型不仅需要[原文此处缺失相关内容]，还需要通过转座子诱变鉴定出的大量“辅助基因”。大多数辅助突变体在含有低于最低抑菌浓度的莫匹罗星的情况下生长时，显示出对苯唑西林的耐药水平大幅提高，这表明辅助突变体耐药性降低的机制涉及严格应激反应的中断，导致青霉素结合蛋白2A（PBP 2A）的产生减少。
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