Rahimi Hamid Reza, Mohammadzadeh Tahereh, Sadjjadi Seyed Mahmoud, Sarkari Bahador, Zahabiun Farzaneh
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Immunol. 2017 Jun;14(2):123-133.
Echinococcosis is a zoonotic parasitic disease caused by the larval stage of Echinococcus granulosus. Several native and recombinant antigens, derived from different stages of E. granulosus life cycle, have been used for vaccine trials. In vitro reared adult worms are good candidates for vaccination as they do not produce fertile egg/s and do not have any risk of contamination for researchers.
To evaluate different antigens derived from in vitro reared E. granulosus adult worms for the immunization of BALB/c mice against secondary hydatidosis.
Viable protoscoleces (PCSs) of sheep hydatid cyst were cultivated in S.10E.H media. Excretory secretory (E/S) and crude antigens were prepared from reared adult worms. A total of fifty BALB/c mice, each 8-weeks-old, were divided into 5 groups of 10 mice. Three groups were subcutaneously immunized with crude, E/S and immunodominant antigens on days 1 and 28. The fourth group received only PBS and the fifth group had no injection. Three weeks following the second immunization, all groups were challenged, intraperitoneal, with viable PSCs. After the autopsy of the mice and opening their abdominal wall, cysts were counted and measured followed by histopathological observations.
The highest protective immunity (98.7%) against hydatidosis was induced by crude antigen, followed by E/S and immunodominant antigens.
Antigens (crude antigens in particular) derived from in vitro reared E. granulosus adult worms, and their different protein components are suitable candidates for the vaccination of intermediate hosts against hydatidosis.
包虫病是由细粒棘球绦虫幼虫阶段引起的一种人畜共患寄生虫病。来自细粒棘球绦虫生命周期不同阶段的几种天然和重组抗原已用于疫苗试验。体外饲养的成虫是疫苗接种的良好候选对象，因为它们不产生可育卵，且对研究人员没有任何污染风险。
评估体外饲养的细粒棘球绦虫成虫来源的不同抗原对BALB/c小鼠继发性包虫病的免疫效果。
将绵羊包虫囊肿的活原头蚴在S.10E.H培养基中培养。从饲养的成虫中制备排泄分泌（E/S）抗原和粗抗原。将总共50只8周龄的BALB/c小鼠分为5组，每组10只。三组分别在第1天和第28天皮下注射粗抗原、E/S抗原和免疫显性抗原。第四组仅接受磷酸盐缓冲液（PBS），第五组不进行注射。第二次免疫后3周，所有组腹腔注射活原头蚴进行攻击。小鼠解剖并打开腹壁后，对囊肿进行计数和测量，随后进行组织病理学观察。
粗抗原诱导的抗包虫病保护性免疫最高（98.7%），其次是E/S抗原和免疫显性抗原。
体外饲养的细粒棘球绦虫成虫来源的抗原（尤其是粗抗原）及其不同的蛋白质成分是中间宿主预防包虫病疫苗接种的合适候选物。
