Rahimi Hamid Reza, Sarkari Bahador, Mohammadzadeh Tahereh, Sadjjadi Seyed Mahmoud
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, e-mail:
Iran J Immunol. 2011 Dec;8(4):236-43.
Cystic echinococcosis (CE), also known as echinococcosis/hydatidosis, is one of the most important parasitic diseases in the world. It enhances both humoral and cellular (Th1 and Th2) responses in infected host. Different antigens of the worm may favor the Th1 or Th2 immune responses in CE patients.
To evaluate the humoral and cellular immune responses of Balb/c mice against the crude and excretory/secretory (E/S) antigens of in vitro reared Echinococcus granulosus adult worms.
A total of 20 Balb/c mice divided into 5 groups of 4 mice each. Three groups of mice (n=4) were immunized with crude, E/S and an immunodominant antigen of in vitro reared Echinococcus granulosus adult worms on day 1 and 28. The fourth and the fifth groups were negative control groups and received PBS plus adjuvant, or nothing, respectively. Two weeks after the second injection, the mice were killed and their blood was collected for determining antibody responses, and their spleens were employed for proliferation assay. Total IgG was measured by indirect ELISA. Spleen cells of immunized mice were cultivated and exposed to different antigens of adult worms including E/S and crude antigens. Levels of IFN-γ, IL-12, IL-4 and IL-10 were measured in the recovered cell culture supernatants by capture ELISA.
Total IgG assay showed the highest level of antibody produced in mice immunized with crude antigens. Proliferation assay showed a statistically significant production of cytokines in the mice immunized with crude antigens (p<0.05). The highest levels of IFN-γ, IL-12 and IL-4 were produced in mice immunized with crude antigen of the in vitro reared Echinococcus granulosus adult worms followed by E/S antigens. Immunodomonant antigen induced the lowest levels of cytokines (IL-12, IFN-γ, IL-4 and IL-10) in immunized mice.
Significant levels of Th1 related cytokines (IFN-γ and IL-12) were produced in Balb/c mice immunized with crude antigen of the in vitro reared Echinococcus granulosus adult worms.
囊性棘球蚴病（CE），也称为棘球蚴病/包虫病，是世界上最重要的寄生虫病之一。它可增强受感染宿主的体液免疫和细胞免疫（Th1和Th2）反应。该蠕虫的不同抗原可能有利于CE患者的Th1或Th2免疫反应。
评估Balb/c小鼠针对体外培养的细粒棘球绦虫成虫粗抗原和排泄/分泌（E/S）抗原的体液免疫和细胞免疫反应。
将20只Balb/c小鼠分为5组，每组4只。三组小鼠（n = 4）在第1天和第28天分别用体外培养的细粒棘球绦虫成虫的粗抗原、E/S抗原和一种免疫显性抗原进行免疫。第四组和第五组为阴性对照组，分别接受PBS加佐剂或不接受任何处理。第二次注射两周后，处死小鼠并采集血液以测定抗体反应，取脾脏进行增殖试验。通过间接ELISA测定总IgG。培养免疫小鼠的脾细胞，并使其接触成虫的不同抗原，包括E/S抗原和粗抗原。通过捕获ELISA测定回收的细胞培养上清液中IFN-γ、IL-12、IL-4和IL-10的水平。
总IgG测定显示，用粗抗原免疫的小鼠产生的抗体水平最高。增殖试验显示，用粗抗原免疫的小鼠中细胞因子的产生具有统计学意义（p < 0.05）。用体外培养的细粒棘球绦虫成虫粗抗原免疫的小鼠产生的IFN-γ、IL-12和IL-4水平最高，其次是E/S抗原。免疫显性抗原在免疫小鼠中诱导产生的细胞因子（IL-12、IFN-γ、IL-4和IL-10）水平最低。
用体外培养的细粒棘球绦虫成虫粗抗原免疫的Balb/c小鼠产生了显著水平的Th1相关细胞因子（IFN-γ和IL-12）。
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