DRD4基因多态性作为伊朗人群中注意缺陷多动障碍儿童的一个风险因素
DRD4 Gene Polymorphisms as a Risk Factor for Children with Attention Deficit Hyperactivity Disorder in Iranian Population.
作者信息
Tabatabaei Seyed Mahmoud, Amiri Shahrokh, Faghfouri Sara, Noorazar Seyed Gholamreza, AbdollahiFakhim Shahin, Fakhari Ali
机构信息
Department of Physiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Research Center of Psychiatry and Behavioral Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Int Sch Res Notices. 2017 May 24;2017:2494537. doi: 10.1155/2017/2494537. eCollection 2017.
BACKGROUND AND OBJECTIVE
Dopamine dysfunction is known to be associated with attention deficit hyperactivity disorder (ADHD). Dopamine D4 receptor gene (DRD4) is one of the important genes in this pathway. This study intended to investigate the variable number of tandem repeats (VNTR) in exon 3 of the DRD4 gene in Iranian children and adolescents.
MATERIALS AND METHODS
In this study, 130 children with ADHD, aged 6-14 years, and 130 healthy children, within the same age range, were enrolled. All children were selected from northwest of Iran which have Caucasian ethnic background and are of a Turkic ethnic group. VNTR polymorphisms of the DRD4 gene were evaluated by PCR using exon 3-specific primers followed by agarose gel electrophoresis.
FINDINGS
The Hardy-Weinberg principle and Chi-square test showed a significant difference in 4-repetition (4R) alleles between the ADHD (76.2%) and control (53.8%) groups ( = 0.004; = 17.39; df = 5). The least percentage of repetition alleles in both groups was 2R.
CONCLUSION
There is a significant correlation between the 4R alleles of DRD4 and ADHD in the northwest of Iran.
背景与目的
已知多巴胺功能障碍与注意力缺陷多动障碍（ADHD）有关。多巴胺D4受体基因（DRD4）是该通路中的重要基因之一。本研究旨在调查伊朗儿童和青少年DRD4基因外显子3中串联重复序列（VNTR）的可变数目。
材料与方法
本研究纳入了130名6至14岁的ADHD儿童和130名年龄范围相同的健康儿童。所有儿童均选自伊朗西北部，具有高加索人种背景且属于突厥族群。使用外显子3特异性引物通过聚合酶链反应（PCR）评估DRD4基因的VNTR多态性，随后进行琼脂糖凝胶电泳。
研究结果
哈迪-温伯格原理和卡方检验显示，ADHD组（76.2%）和对照组（53.8%）之间的4重复（4R）等位基因存在显著差异（P = 0.004；χ² = 17.39；自由度 = 5）。两组中重复等位基因比例最低的是2R。
结论
在伊朗西北部，DRD4基因的4R等位基因与ADHD之间存在显著相关性。
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