Zhou Guowei, Cai Lin, Yuan Tao, Tian Renmao, Tong Haoya, Zhang Weipeng, Jiang Lei, Guo Minglan, Liu Sheng, Qian Pei-Yuan, Huang Hui
Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
Tropical Marine Biological Research Station in Hainan, Chinese Academy of Sciences, Sanya, Hainan, China.
Environ Microbiol. 2017 Aug;19(8):3342-3352. doi: 10.1111/1462-2920.13840. Epub 2017 Jul 24.
Reef-building corals are complex holobionts, harbouring diverse microorganisms that play essential roles in maintaining coral health. However, microbiome development in early life stages of corals remains poorly understood. Here, microbiomes of Acropora gemmifera were analysed during spawning and early developmental stages, and also under different seawater partial pressure of CO (pCO ) conditions, using amplicon sequencing of 16S rRNA gene for bacteria and archaea and of ITS2 for Symbiodinium. No remarkable microbiome shift was observed in adults before and after spawning. Moreover, microbiomes in eggs were highly similar to those in spawned adults, possibly suggesting a vertical transmission from parents to offspring. However, significant stage-specific changes were found in coral microbiome during development, indicating that host development played a dominant role in shaping coral microbiome. Specifically, Cyanobacteria were particularly abundant in 6-day-old juveniles, but decreased largely in 31-day-old juveniles with a possible subclade shift in Symbiodinium dominance from C2r to D17. Larval microbiome showed changes in elevated pCO , while juvenile microbiomes remained rather stable in response to higher pCO . This study provides novel insights into the microbiome development during the critical life stages of coral.
造礁珊瑚是复杂的共生体，其体内栖息着多种微生物，这些微生物在维持珊瑚健康方面发挥着重要作用。然而，珊瑚早期生命阶段的微生物群落发育仍知之甚少。在此，利用对细菌和古菌的16S rRNA基因以及对共生藻的ITS2进行扩增子测序，分析了宝石鹿角珊瑚在产卵期和早期发育阶段，以及在不同海水二氧化碳分压（pCO₂）条件下的微生物群落。在产卵前后的成体中未观察到明显的微生物群落变化。此外，卵中的微生物群落与产卵后的成体高度相似，这可能表明存在从亲代到子代的垂直传播。然而，在珊瑚发育过程中发现了珊瑚微生物群落显著的阶段特异性变化，这表明宿主发育在塑造珊瑚微生物群落中起主导作用。具体而言，蓝细菌在6日龄幼体中特别丰富，但在31日龄幼体中大量减少，共生藻优势可能从C2r亚分支转变为D17亚分支。幼体微生物群落在pCO₂升高时出现变化，而幼体微生物群落在pCO₂升高时保持相对稳定。本研究为珊瑚关键生命阶段的微生物群落发育提供了新的见解。
