Pharmaceutical Analysis Research Center, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Science, Tabriz, Iran.
J Sep Sci. 2017 Aug;40(16):3318-3326. doi: 10.1002/jssc.201700385. Epub 2017 Jul 17.
A dispersive micro-solid-phase extraction procedure coupled with capillary electrophoresis ultraviolet detection was developed for determination of verapamil in plasma samples. Graphene oxide/polydopamin was synthesized by a one-step polymerization method, and graphene oxide/Fe O (magnetic graphene oxide) nanocomposite was prepared by coprecipitation method. Moreover, they were fully characterized. The use of hazardous and water-immiscible solvents was scaled down, and only 500 μL of acetone was required as the desorption solvent. The detector response concentration plots were linear in the range of 5-500 ng/mL, and the proposed method was validated according to guidelines. The precision and accuracy were less than 15%. Dispersive micro-solid-phase extraction method provides a rapid, environmentally friendly, and sensitive analysis for the verapamil in patient plasma samples, which is adequate for therapeutic drug monitoring and pharmacokinetic studies.
建立了一种分散微固相萃取-毛细管电泳紫外检测法，用于测定血浆样品中的维拉帕米。采用一步聚合法合成了氧化石墨烯/聚多巴胺，并用共沉淀法制备了氧化石墨烯/Fe3O4（磁性氧化石墨烯）纳米复合材料，并对其进行了充分的表征。减少了使用危险和不混溶溶剂，仅需 500μL 丙酮作为洗脱溶剂。在 5-500ng/mL 的范围内，检测器的响应浓度呈线性关系，且该方法是根据指南进行验证的。精密度和准确度均小于 15%。分散微固相萃取法为患者血浆样品中的维拉帕米提供了一种快速、环保、灵敏的分析方法，适用于治疗药物监测和药代动力学研究。
