Food and Drug Safety Research Center, Tabriz University of Medical Science, Tabriz, Iran.
Department of Organic Chemistry, Azarbaijan Shahid Madani University, Tabriz, Iran.
Asian Pac J Cancer Prev. 2020 Jun 1;21(6):1531-1538. doi: 10.31557/APJCP.2020.21.6.1531.
For biological assessing of hospital personnel occupationally exposed to antineoplastic drugs, highly sensitive and accurate methods are required. Methotrexate (MTX) is an anticancer agent that is widely used in a variety of human cancers. For the first time, dispersive-micro solid phase extraction (D-µ-SPE) has been applied for determination of low levels of MTX in saliva samples. The method is based on rapid extraction of MTX using graphene oxide adsorbent. The sample preparation time is decreased by the fact that the adsorbent dispersed in the sample solution and extraction equilibrium can be reached very fast. This significant feature which obtained with this method is of key interest for routine trace laboratory analysis. The influence of different variables on D-µ-SPE was investigated. Under optimum conditions, the calibration graph was linear over the range of 10-1000 ng/ml. The relative standard deviations are better than 9.0%. The proposed method was successfully applied for the determination of MTX in patient samples.
为了对职业接触抗肿瘤药物的医院人员进行生物学评估，需要使用高度敏感和准确的方法。甲氨蝶呤（MTX）是一种广泛用于多种人类癌症的抗癌药物。首次应用分散-微固相萃取（D-µ-SPE）法测定唾液中甲氨蝶呤的低浓度。该方法基于使用氧化石墨烯吸附剂快速提取 MTX。由于吸附剂分散在样品溶液中，并且可以非常快速地达到萃取平衡，因此可以缩短样品制备时间。这种方法的显著特点对于常规痕量实验室分析至关重要。研究了不同变量对 D-µ-SPE 的影响。在最佳条件下，校准曲线在 10-1000ng/ml 范围内呈线性。相对标准偏差优于 9.0%。该方法成功地应用于患者样本中甲氨蝶呤的测定。
