Liu Tiansheng, Ke Fushi, You Shijun, Chen Wenbin, He Weiyi, You Minsheng
Institute of Applied Ecology and Research Centre for Biodiversity and Eco-Safety, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Insects. 2017 Jun 20;8(2):63. doi: 10.3390/insects8020063.
Fourteen polymorphic microsatellite loci were isolated in this transcriptome-based data analysis for , which is an important larval parasitoid of the worldwide pest . A subsequent test was performed for a wild population ( = 32) from Fuzhou, Fujian, southeastern China, to verify the effectiveness of the 14 microsatellite loci in future studies on genetic diversity. The observed number of alleles ranged from two to six. The expected and observed heterozygosity ranged from 0.123 to 0.316 and from 0.141 to 0.281, respectively. The polymorphism information content () value ranged from 0.272 to 0.622. Potentially due to the substructure of the sampled population, three of the 14 microsatellite loci deviated from Hardy-Weinberg equilibrium (). Further, loci C6, C22, and C31 could be amplified in and , suggesting the transferability of these three polymorphic loci to other species of Hymenoptera.
在基于转录组的数据分析中，分离出了14个多态性微卫星位点， 是一种重要的幼虫寄生蜂，寄生于全球范围内的害虫 。随后，对来自中国东南部福建福州的一个野生 种群（n = 32）进行了测试，以验证这14个微卫星位点在未来 遗传多样性研究中的有效性。观察到的等位基因数量从2到6不等。预期杂合度和观察到的杂合度分别在0.123至0.316和0.141至0.281之间。多态性信息含量（PIC）值在0.272至0.622之间。可能由于抽样群体的亚结构，14个微卫星位点中的3个偏离了哈迪-温伯格平衡（HWE）。此外，位点C6、C22和C31可以在 和 中扩增，这表明这三个多态性位点可转移到其他膜翅目物种。
