葡萄实蝇（Pachycrepoideus vindemmiae (Rondani)，膜翅目：金小蜂科）多态微卫星位点的分离与特征分析

Isolation and characterization of polymorphic microsatellite loci for Pachycrepoideus vindemmiae (Rondani) (Hymenoptera: Pteromalidae).

作者信息

Chen W, Fang L, Liu J L, He Z, Hu H Y

机构信息

Key Laboratory of Biotic Environment and Ecological Safety, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.

Key Laboratory of Biotic Environment and Ecological Safety, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China

出版信息

Genet Mol Res. 2015 Mar 13;14(1):1798-801. doi: 10.4238/2015.March.13.7.

DOI:10.4238/2015.March.13.7

PMID:25867324

Abstract

The parasitoid wasp Pachycrepoideus vindemmiae (Rondani) is a common pupal parasitoid of many fly pests that is distributed worldwide. This organism can be used for biological control in orchards or livestock farms. Identifying polymorphic microsatellite loci would be useful for analyzing the population genetic structure of the parasitoid. In the current study, based on a modified biotin-capture method, 10 polymorphic microsatellite loci were isolated and characterized for the insect, 7 of which did not deviate from Hardy-Weinberg equilibrium. The allelic number per locus varied from 3-7 (N = 30). The expected and observed heterozygosities of 10 loci ranged from 0.369-0.775 and from 0.300-0.867, respectively.

摘要

寄生蜂黄足扁股小蜂（Pachycrepoideus vindemmiae (Rondani)）是许多蝇类害虫常见的蛹期寄生蜂，分布于世界各地。这种生物可用于果园或畜牧场的生物防治。鉴定多态性微卫星位点有助于分析该寄生蜂的种群遗传结构。在本研究中，基于改良的生物素捕获方法，为该昆虫分离并鉴定了10个多态性微卫星位点，其中7个位点未偏离哈迪-温伯格平衡。每个位点的等位基因数在3 - 7之间（N = 30）。10个位点的预期杂合度和观察到的杂合度分别在0.369 - 0.775和0.300 - 0.867之间。