Takuwa Y, Ogata E
In Vitro Cell Dev Biol. 1985 Aug;21(8):445-9. doi: 10.1007/BF02620832.
The JTC-12 cell, an established cell line derived from a normal monkey kidney, was studied in an attempt to characterize the epithelial qualities. Phase contrast microscopy showed dome formation in confluent monolayers and electron microscopic examinations revealed the presence of numerous microvilli on the apical membranes and desmosome between cells. Sonicated cells showed activities of gamma-glutamyl transpeptidase, leucine aminopeptidase, alkaline phosphatase, and trehalase, marker enzymes of renal proximal epithelium. Alkaline phosphatase activity exhibited the characteristics of a renal type isozyme. Furthermore, confluent JTC-12 monolayers exhibited Na+-dependent transport of hexose, amino acid as well as inorganic phosphate. These findings indicate that JTC-12 cells in monolayer culture maintain ultrastructural, biochemical, and physiological properties of renal proximal epithelial cells. This cell line will be useful for further studies on cellular functions of renal proximal epithelium.
JTC - 12细胞是一种源自正常猴肾的成熟细胞系，为了表征其上皮细胞特性而对其进行了研究。相差显微镜观察显示汇合的单层细胞形成了穹顶，电子显微镜检查发现顶端膜上存在大量微绒毛以及细胞间的桥粒。超声处理后的细胞显示出γ-谷氨酰转肽酶、亮氨酸氨肽酶、碱性磷酸酶和海藻糖酶的活性，这些都是肾近端上皮细胞的标记酶。碱性磷酸酶活性表现出肾型同工酶的特征。此外，汇合的JTC - 12单层细胞表现出对己糖、氨基酸以及无机磷酸盐的钠依赖性转运。这些发现表明单层培养的JTC - 12细胞保持了肾近端上皮细胞的超微结构、生化和生理特性。该细胞系将有助于进一步研究肾近端上皮细胞的细胞功能。
