Higuchi S, Urano C, Kawamura S
J Chromatogr. 1985 Jun 14;341(2):305-11. doi: 10.1016/s0378-4347(00)84044-x.
A highly sensitive method for the determination of propafenone in plasma has been established using gas chromatography-mass spectrometry with the deuterium-labelled compound as an internal standard. Plasma levels as low as 1 ng/ml were measured using 0.5-ml plasma samples. Plasma protein binding of the drug in rats, dogs and humans in vitro and in vivo was determined by the proposed method. About 90% of the drug was bound to the plasma protein in all species in vitro (20-500 ng/ml) and 69-88% in rats, 90-95% in dogs and 77-89% in humans after oral administration of the drug at doses of 50 mg/kg, 5 mg/kg and 200 mg per person, respectively.
已建立一种高灵敏度的血浆中普罗帕酮测定方法，该方法采用气相色谱 - 质谱联用，以氘标记化合物作为内标。使用0.5毫升血浆样本可测定低至1纳克/毫升的血浆水平。通过该方法测定了该药物在大鼠、犬和人体的体外及体内血浆蛋白结合率。在体外（20 - 500纳克/毫升），所有物种中约90%的药物与血浆蛋白结合；分别以50毫克/千克、5毫克/千克的剂量给大鼠和犬口服给药，以及以每人200毫克的剂量给人体口服给药后，大鼠体内的结合率为69 - 88%，犬为90 - 95%，人体为77 - 89%。
