Med Princ Pract. 2017;26(4):375-380. doi: 10.1159/000478782. Epub 2017 Jun 19.
To detect urinary volatile organic compounds (VOCs) in patients with idiopathic membranous nephropathy (iMN) and normal controls, and to examine whether or not urinary VOCs can act as biomarkers for the diagnosis of iMN independent of renal biopsy.
Gas chromatography/mass spectrometry (GC/MS) was used to assess the urine collected from 63 iMN patients and 15 normal controls. The statistical methods of principal component analysis and partial least squares discriminant analysis were performed to process the final data in Common Data Format which were converted from GC/MS data.
Six VOCs in the urine samples of iMN patients exhibited significant differences from those of normal controls: carbamic acid monoammonium salt, 2-pentanone, 2,4-dimethyl-pentanal, hydrogen azide, thiourea, and 4-heptanone were significantly higher than in controls (p < 0.05).
Six urinary VOCs were isolated from patients with iMN using GC/MS. The analysis of urinary VOCs using GC/MS could be developed into a non-invasive method for the detection of iMN.
检测特发性膜性肾病（iMN）患者和正常对照者的尿挥发性有机化合物（VOCs），并探讨尿 VOCs 是否可作为独立于肾活检的 iMN 诊断的生物标志物。
采用气相色谱/质谱（GC/MS）法检测 63 例 iMN 患者和 15 例正常对照者的尿液。采用主成分分析和偏最小二乘判别分析等统计方法对从 GC/MS 数据转换而来的通用数据格式的最终数据进行处理。
iMN 患者尿液中的 6 种 VOCs 与正常对照组相比有显著差异：氨基甲酸单铵盐、2-戊酮、2,4-二甲基戊醛、叠氮化氢、硫脲和 4-庚酮均明显高于对照组（p＜0.05）。
采用 GC/MS 从 iMN 患者尿液中分离出 6 种尿 VOCs。GC/MS 分析尿 VOCs 可能成为一种非侵入性检测 iMN 的方法。
