Bone Regenerative Engineering Laboratory, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.
Toyobo Co. Ltd, Research Institute, Shiga, Japan.
J Tissue Eng Regen Med. 2018 Feb;12(2):e1186-e1194. doi: 10.1002/term.2505. Epub 2017 Oct 12.
An octacalcium phosphate and collagen composite (OCP/Col) achieved efficient bone regeneration with excellent resorbability. After the confirmation of its safety and efficacy in preclinical animal studies, the present study investigated the influence of electron beam irradiation doses on bone regeneration by OCP/Col in order to secure its commercialization. OCP/Col was prepared as previously described and the packed OCP/Col was sterilized using different doses of electron beam irradiation. A standardized defect, 9 mm in diameter, was made in the rat calvarium. A disk of OCP/Col (diameter 9 mm, thickness 1.5 mm) sterilized using different doses of electron beam irradiation was then implanted into the defect. Five defects in each group were treated and fixed 4, 12 and 24 weeks after implantation. Specimens were decalcified and stained with haematoxylin and eosin. In a histomorphometrical analysis, the percentage of newly formed bone in the defect (n-Bone%) was calculated. In OCP/Col 15 kGy, newly formed bone was enhanced and present throughout the defect with the resorption of OCP/Col. Furthermore, vigorous bone remodelling and bone maturation were observed. In OCP/Col 40 kGy, newly formed bone was not as prominent as that with OCP/Col 15 kGy. A histomorphometrical analysis using Student's t-test at 24 weeks revealed that the n-Bone% of OCP/Col 15 kGy (65.9 ± 7.14%) was significantly higher than that of OCP/Col 40 kGy (38.0 ± 10.2%). These results suggest that different electron beam irradiation doses influence bone regeneration by OCP/Col.
一种八钙磷和胶原复合材料（OCP/Col）具有优异的可吸收性，实现了高效的骨再生。在临床前动物研究中确认其安全性和有效性后，本研究旨在为确保其商业化，调查 OCP/Col 中电子束照射剂量对骨再生的影响。OCP/Col 按先前的描述制备，并用不同剂量的电子束照射对 OCP/Col 进行灭菌。在大鼠颅骨上制作直径为 9mm 的标准化缺损。将用不同剂量电子束照射灭菌的直径 9mm、厚度 1.5mm 的 OCP/Col 圆盘植入到缺损处。每组 5 个缺损，在植入后 4、12 和 24 周进行处理和固定。将标本脱钙并用苏木精和伊红染色。在组织形态计量学分析中，计算缺陷中新形成骨的百分比（n-Bone%）。在 OCP/Col 15kGy 中，新形成的骨增强，并在 OCP/Col 吸收的过程中遍布整个缺损。此外，观察到强烈的骨重塑和骨成熟。在 OCP/Col 40kGy 中，新形成的骨不如 OCP/Col 15kGy 明显。在 24 周时使用学生 t 检验进行的组织形态计量学分析表明，OCP/Col 15kGy 的 n-Bone%（65.9±7.14%）明显高于 OCP/Col 40kGy（38.0±10.2%）。这些结果表明，不同的电子束照射剂量会影响 OCP/Col 的骨再生。
