Korstanje Ron, Ryan Jennifer L, Savage Holly S, Lyons Bonnie L, Kane Kevin G, Sukoff Rizzo Stacey J
The Jackson Laboratory, Bar Harbor, Maine 04609.
Endocrinology. 2017 Sep 1;158(9):2707-2712. doi: 10.1210/en.2017-00266.
Previous studies with continuous glucose monitoring in mice have been limited to several days or weeks, with the mouse's physical attachment to the equipment affecting behavior and measurements. In the current study, we measured blood glucose and body temperature at 10-second intervals for 12 weeks in a cohort of NOD/ShiLtJ female mice using wireless telemetry. This allowed us to obtain a high-resolution profile of the circadian rhythm of these two parameters and the onset of hyperglycemic development in real time. The most striking observations were the elevated nocturnal concentrations of glucose into the diabetic range days before elevations in diurnal glucose (when glucose concentrations are historically measured) and the strong, negative correlation between elevated blood glucose concentrations and body temperature with a steady decline of the body temperature with diabetes development. Taken together, this technological advancement provides improved resolution in the study of the disease trajectory of diabetes in mouse models, including relevant translatability to the current technologies of continuous glucose monitoring now regularly used in patients.
先前对小鼠进行连续血糖监测的研究仅限于几天或几周，小鼠与设备的物理连接会影响行为和测量结果。在本研究中，我们使用无线遥测技术，对一组NOD/ShiLtJ雌性小鼠进行了为期12周的测量，每隔10秒测量一次血糖和体温。这使我们能够实时获得这两个参数昼夜节律的高分辨率概况以及高血糖发展的起始情况。最显著的观察结果是，在日间血糖升高（传统上测量血糖浓度的时间）前几天，夜间血糖浓度升高至糖尿病范围，并且血糖浓度升高与体温之间存在强烈的负相关，随着糖尿病的发展体温持续下降。综上所述，这一技术进步为小鼠模型中糖尿病疾病轨迹的研究提供了更高的分辨率，包括与目前患者经常使用的连续血糖监测现有技术的相关可转化性。
