Adikusuma Fatwa, Williams Nicole, Grutzner Frank, Hughes James, Thomas Paul
School of Biological Science, The University of Adelaide, Adelaide, SA 5005, Australia; Centre for Biomedical Research, Faculty of Medicine, Diponegoro University, Semarang 50271, Indonesia.
School of Biological Science, The University of Adelaide, Adelaide, SA 5005, Australia.
Mol Ther. 2017 Aug 2;25(8):1736-1738. doi: 10.1016/j.ymthe.2017.05.021. Epub 2017 Jun 17.
CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes.
CRISPR/Cas9基因组编辑技术能够促进基因组区域的高效删除，但尚未被用于删除整条染色体。在此，阿迪库苏马等人通过去除小鼠胚胎干细胞和受精卵中的着丝粒或切割染色体臂，展示了CRISPR介导的高效选择性染色体删除的概念验证。
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