Poursani Ensieh M, Mehravar Majid, Soltani Bahram Mohammad, Mowla Seyed Javad
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Gene. 2017 Sep 5;627:369-372. doi: 10.1016/j.gene.2017.06.032. Epub 2017 Jun 17.
POU domain proteins are an important family of transcription factors that regulates cell type-specific gene expression. One of the most crucial members of this family that maintains pluripotency and self-renewal of embryonic stem cells is POU5F1/OCT4. The OCT4 gene can generate several variants under different situations/cell types includes OCT4A that is the major factor sustains pluripotency in embryonic stem and embryonic carcinoma cells, and also OCT4B and OCT4B1, which are transcribed from a different potential promoter located in intron1 and are expressed in various tissues and cell types. In present study, during expression check of OCT4B1 in embryonic carcinoma cells (NT2), we discovered a novel OCT4 transcript for the first time and designated it as OCT4B4. This variant is expressed in various human pluripotent cells and its expression is down-regulated upon induction of differentiation. Moreover, knocking down of OCT4B4 by shRNA resulted in increased accumulation of transfected cells in G0/G1 phase compared to the mock-transfected control cells.
POU结构域蛋白是一类重要的转录因子家族，可调节细胞类型特异性基因表达。该家族中维持胚胎干细胞多能性和自我更新的最关键成员之一是POU5F1/OCT4。OCT4基因在不同情况/细胞类型下可产生多种变体，包括OCT4A，它是维持胚胎干细胞和胚胎癌细胞多能性的主要因子，还有OCT4B和OCT4B1，它们从位于内含子1中的不同潜在启动子转录而来，并在各种组织和细胞类型中表达。在本研究中，在检测胚胎癌细胞（NT2）中OCT4B1的表达时，我们首次发现了一种新的OCT4转录本，并将其命名为OCT4B4。这种变体在各种人类多能细胞中表达，并且在诱导分化时其表达下调。此外，与模拟转染的对照细胞相比，用shRNA敲低OCT4B4导致转染细胞在G0/G1期的积累增加。
